School of Biotechnology, KIIT University, Bhubaneswar, Odisha, 751024, India.
School of Law, KIIT University, Bhubaneswar, Odisha, 751024, India.
J Microbiol. 2018 Apr;56(4):223-230. doi: 10.1007/s12275-018-7387-x. Epub 2018 Apr 2.
moderately halophilic spore forming, motile, Gram-positive, rod-shaped bacterial strain designated as KGW1 was isolated from water sample of Chilika Lake and characterized taxonomically using polyphasic approach. The strain grew in the presence of 0-25% (w/v) NaCl in marine salt agar media, hydrolyzes casein, and gelatin and shows presence of alkaline proteases. The major cell wall menaquinone was MK7 and major cellular fatty acids were anteiso-C (44.89%), anteiso-C (6.18%), isoC (19.38%), and iso-C (7.39%). Several chemotaxonomic features conform the isolate be a member of genus Halobacillus. The isolate KGW1 contained A1γ meso-Dpm-direct type of peptidoglycan which is different from its phylogenetically closest neighbours. The 16S rRNA gene sequence based phylogenetic analysis also revealed the strain KGW1 was affiliated to the genus Halobacillus and sequence similarity between the isolated strain and the type strains of Halobacillus species were found closest to, H. dabanensis D-8 DSM 18199 (99.08%) and H. faecis IGA7-4 DSM 21559 (99.01%), H. trueperi SL-5 DSM 10404 (98.94%). The in silico DDH showed that the values in a range of 14.2-17.5% with the most closest strain H. dabanensis D-8 DSM 18199 and other type strains of the genus Halobacillus for which whole genome sequence is reported. DNA-DNA relatedness between strain KGW1 and the closest type strain Halobacillus trueperi DSM 10404 was 11.75% (± 1.15). The draft genome sequence includes 3,683,819 bases and comprises of 3898 predicted coding sequences with a G + C content of 46.98%. Thus, the significant distinctiveness supported by phenotypic and genotypic data with its closest neighbors and other closely related species confirm the strain KGW1 to be classified as a novel species within the genus Halobacillus, for which the name Halobacillus marinus sp. nov. is proposed. The type strain is KGW1 (= DSM 29522 = JCM 30443).
从奇利卡湖水样中分离到一株中度嗜盐、能运动、革兰氏阳性、杆状的细菌菌株，命名为 KGW1，并采用多相分类法对其进行了分类学研究。该菌株在含有 0-25%（w/v）NaCl 的海水盐琼脂培养基中生长，可水解酪蛋白和明胶，并显示碱性蛋白酶的存在。主要的细胞壁menaquinone 为 MK7，主要的细胞脂肪酸为anteiso-C（44.89%）、anteiso-C（6.18%）、isoC（19.38%）和 iso-C（7.39%）。一些化学生态分类学特征表明，该分离株是 Halobacillus 属的成员。分离株 KGW1 含有 A1γ meso-Dpm-direct 型肽聚糖，与亲缘关系最近的菌株不同。基于 16S rRNA 基因序列的系统发育分析也表明，菌株 KGW1 属于 Halobacillus 属，与 Halobacillus 种的模式菌株的序列相似性最接近，分别为 H. dabanensis D-8 DSM 18199（99.08%）和 H. faecis IGA7-4 DSM 21559（99.01%），H. trueperi SL-5 DSM 10404（98.94%）。在模拟 DNA-DNA 杂交试验中，与最接近的菌株 H. dabanensis D-8 DSM 18199 和其他已报道全基因组序列的 Halobacillus 属的模式菌株的数值在 14.2-17.5%之间。菌株 KGW1 与最接近的模式菌株 Halobacillus trueperi DSM 10404 的 DNA-DNA 相关性为 11.75%（±1.15）。基因组序列草图包含 3,683,819 个碱基，包含 3898 个预测编码序列，G+C 含量为 46.98%。因此，表型和基因型数据与最接近的近缘种和其他密切相关的种显著不同，证实了菌株 KGW1 可被分类为 Halobacillus 属的一个新种，命名为 Halobacillus marinus sp. nov.。模式菌株为 KGW1（=DSM 29522=JCM 30443）。
