Otani Y, Tabata Y, Ikada Y
Institute for Frontier Medical Sciences, Kyoto University, Japan.
Ann Thorac Surg. 1999 Apr;67(4):922-6. doi: 10.1016/s0003-4975(99)00153-8.
Air leak is a problem commonly occurring in lung and thoracic operations. In this study, a rapidly curable hydrogel glue was prepared as the seal for lung air leak.
Mixing an aqueous solution of gelatin and poly(L-glutamic acid) with a water-soluble carbodiimide produced a hydrogel. The sealing effect on the air leak wound of rat lung was compared with that of conventional fibrin glue.
The gelatin-poly(L-glutamic acid) hydrogel glue was solidified as rapidly as the fibrin glue, and was significantly more effective in sealing the lung air leak than the fibrin glue. Approximately 80% of the lungs treated with the hydrogel glue exhibited no air leak at the lung pressure of 50 cm H2O. Urea addition could prevent spontaneous gelatination of the mixed solution at room temperature and did not affect the hydrogel sealing effect. The bonding strength of the hydrogel glue both with and without urea to the lung tissue was significantly higher than that of the fibrin glue.
We concluded that this strong lung adhesion of the gelatin-poly(L-glutamic acid) hydrogel glue resulted in its superior sealing effect.
漏气是肺部和胸部手术中常见的问题。在本研究中，制备了一种可快速固化的水凝胶胶水作为肺漏气的密封剂。
将明胶水溶液和聚（L-谷氨酸）与水溶性碳二亚胺混合制成水凝胶。将其对大鼠肺漏气伤口的密封效果与传统纤维蛋白胶进行比较。
明胶-聚（L-谷氨酸）水凝胶胶水固化速度与纤维蛋白胶一样快，并且在密封肺漏气方面比纤维蛋白胶显著更有效。在用该水凝胶胶水处理的肺中，约80%在50 cm H₂O的肺压力下无漏气。添加尿素可防止混合溶液在室温下自发凝胶化，且不影响水凝胶的密封效果。含尿素和不含尿素的水凝胶胶水与肺组织的结合强度均显著高于纤维蛋白胶。
我们得出结论，明胶-聚（L-谷氨酸）水凝胶胶水对肺的强粘附性导致其具有优异的密封效果。
