Otani Y, Tabata Y, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
J Biomed Mater Res. 1996 Jun;31(2):158-66. doi: 10.1002/(sici)1097-4636(199606)31:2<157::aid-jbm2>3.0.co;2-m.
This study describes the potentiality of hydrogels composed of gelatin and poly(L-glutamic acid) (PLGA) as a biological glue for soft tissues and compares its effectiveness with that of a conventional fibrin glue. Water-soluble carbodiimides (WSC) were used to crosslink the aqueous mixture of gelatin and PLGA. The mixed aqueous solution of gelatin and PLGA set to a hydrogel by use of WSC as rapidly as BOLHEAL fibrin glue. An addition of PLGA to gelatin aqueous solution reduced not only its gelation time but also the WSC concentration necessary for hydrogel formation. The cured hydrogel exhibited firm adhesion to the mouse skin and other soft tissues with a higher bonding strength than BOLHEAL fibrin glue. Cohesive failure in the hydrogel was observed when the gel-tissue bond was broken, in contrast to BOLHEAL fibrin glue. The bonding strength of the gelatin-PLGA hydrogel became higher with the increasing PLGA concentration. The inflammatory reaction around the gelatin-PLGA hydrogel subcutaneously implanted in mice was mild, and the hydrogel was gradually absorbed with time in vivo. A toxicity test demonstrated that the concentration of WSC necessary as a biological glue was low enough not to induce its toxicity.
本研究描述了由明胶和聚（L-谷氨酸）（PLGA）组成的水凝胶作为软组织生物胶水的潜力，并将其有效性与传统纤维蛋白胶水进行了比较。使用水溶性碳二亚胺（WSC）交联明胶和PLGA的水性混合物。明胶和PLGA的混合水溶液使用WSC时形成水凝胶的速度与BOLHEAL纤维蛋白胶水一样快。向明胶水溶液中添加PLGA不仅缩短了其凝胶化时间，还降低了形成水凝胶所需的WSC浓度。固化后的水凝胶对小鼠皮肤和其他软组织表现出牢固的粘附力，其粘结强度高于BOLHEAL纤维蛋白胶水。与BOLHEAL纤维蛋白胶水不同，当凝胶-组织粘结断裂时，在水凝胶中观察到内聚破坏。明胶-PLGA水凝胶的粘结强度随着PLGA浓度的增加而提高。皮下植入小鼠体内的明胶-PLGA水凝胶周围的炎症反应较轻，并且该水凝胶在体内会随着时间逐渐被吸收。毒性测试表明，作为生物胶水所需的WSC浓度低到足以不引起其毒性。
