Otani Y, Tabata Y, Ikada Y
Research Center for Biomedical Engineering, Kyoto University, Japan.
Biomaterials. 1998 Nov;19(22):2091-8. doi: 10.1016/s0142-9612(98)00121-5.
The hemostatic capability of rapidly curable glues composed of gelatin and poly(L-glutamic acid) (PLGA) was compared with that of the conventional fibrin glue. The hydrogels produced from mixed gelatin and PLGA aqueous solution within several seconds by addition of water-soluble carbodiimide (WSC) was applied to the dog spleen injured by needle pricking. The WSC-catalyzed gelatin-PLGA glues exhibited higher hemostatic capability than the fibrin glue. The total amount of bleeding from the injured spleen until hemostasis when the gelatin-PLGA hydrogel glues were applied was significantly smaller than that of the fibrin glue application. The gelatin-PLGA glue application enhanced the success rate of complete hemostasis to a significantly greater extent than the fibrin glue, while the frequency of glue applications until achieving complete hemostasis decreased. The gelatin PLGA hydrogels strongly adhered to the surface of dog spleen, whereas the fibrin hydrogel was easily detached from the spleen surface. It was concluded that this strong adhesion mechanically suppressed the bleeding, leading to enhanced hemostasis by the rapidly curable gelatin-PLGA glues.
将由明胶和聚（L-谷氨酸）（PLGA）组成的快速固化胶水的止血能力与传统纤维蛋白胶水的止血能力进行了比较。通过添加水溶性碳二亚胺（WSC）在几秒钟内由混合明胶水溶液和PLGA水溶液制成的水凝胶被应用于针刺损伤的犬脾脏。WSC催化的明胶-PLGA胶水比纤维蛋白胶水表现出更高的止血能力。应用明胶-PLGA水凝胶胶水时，受伤脾脏止血前的总出血量明显少于应用纤维蛋白胶水时的出血量。与纤维蛋白胶水相比，应用明胶-PLGA胶水在更大程度上显著提高了完全止血的成功率，同时实现完全止血所需的胶水应用频率降低。明胶-PLGA水凝胶牢固地粘附在犬脾脏表面，而纤维蛋白水凝胶很容易从脾脏表面脱落。得出的结论是，这种强粘附力通过机械方式抑制了出血，从而增强了快速固化的明胶-PLGA胶水的止血效果。
