Li C, Jiang H, Fu Y
Microwave Institute, Zhejiang Medical University, Hangzhou.
Zhonghua Yu Fang Yi Xue Za Zhi. 1998 May;32(3):142-4.
To study the relationship between dose of exposure to 50-Hz magnetic field (MF) and its effects of suppression to gap junctional intercellular communication (GJIC) function.
Lucifer yellow, a kind of fluorescent dye, was led into single CHL cell by iontophoretic injection. Number of dye-coupled cells (DCC) five minutes after injection was used as an indicator for GJIC function. The effects of different intensities and irradiation of MF on GJIC in CHL cells were studied.
Suppression to GJIC by 50-Hz MF depended on its flux intensity. Irradiation with lower flux intensity (0.05, 0.2 and 0.4 mT) of the MF for 24 hours could not inhibit GJIC, but that with higher intensity (0.8 and 1.6 mT) could. No "amplitude window" effect was appeared by irradiation with MF at intensities of zero to 1.6 mT. Exposure to MF at a fixed intensity of 0.8 mT for five minutes had no effects, but that for one hour was more than enough to inhibit GJIC, with the most apparent effects for 24-hour exposure. In addition, it was showed that suppression to GJIC was caused by direct action of 50-Hz MF rather then induced electrical field.
MF of 50-Hz had suppression to GJIC with a dose-effect relationship.
研究50Hz磁场（MF）暴露剂量与其对缝隙连接细胞间通讯（GJIC）功能抑制作用之间的关系。
通过离子电渗注入法将一种荧光染料荧光素黄导入单个CHL细胞。以注入后5分钟时染料偶联细胞（DCC）的数量作为GJIC功能的指标。研究了不同强度和照射时间的MF对CHL细胞中GJIC的影响。
50Hz MF对GJIC的抑制作用取决于其通量强度。较低通量强度（0.05、0.2和0.4mT）的MF照射24小时不能抑制GJIC，但较高强度（0.8和1.6mT）的MF照射则可以。0至1.6mT强度的MF照射未出现“振幅窗口”效应。以0.8mT的固定强度照射MF 5分钟无影响，但照射1小时足以抑制GJIC，24小时照射的抑制效果最明显。此外，结果表明50Hz MF对GJIC的抑制作用是由其直接作用而非感应电场引起的。
50Hz MF对GJIC有抑制作用，且存在剂量效应关系。
