通过对hsp65基因进行PCR-限制性片段长度多态性分析来对麻风分枝杆菌进行种特异性鉴定。
Species-specific identification of Mycobacterium leprae by PCR-restriction fragment length polymorphism analysis of the hsp65 gene.
作者信息
Rastogi N, Goh K S, Berchel M
机构信息
Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, 97165 Pointe à Pitre Cedex, Guadeloupe.
出版信息
J Clin Microbiol. 1999 Jun;37(6):2016-9. doi: 10.1128/JCM.37.6.2016-2019.1999.
Abstract
PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene present in all mycobacteria was used in the present investigation to characterize Mycobacterium leprae. Bacilli were extracted and purified from different organs from experimentally infected armadillos and nude mice (Swiss mice of nu/nu origin). A total of 15 samples were assayed in duplicate, and the results were compared with those obtained for a total of 147 cultivable mycobacteria representing 34 species. Irrespective of its origin or viability, M. leprae strains from all the samples were uniformly characterized by two fragments of 315 and 135 bp upon BstEII digestion and two fragments of 265 and 130 bp upon HaeIII digestion. PRA is a relatively simple method and permits the conclusive identification of M. leprae to the species level.
摘要
本研究采用对所有分枝杆菌中存在的hsp65基因进行聚合酶链反应-限制性片段长度多态性分析（PRA）来鉴定麻风分枝杆菌。从实验感染的犰狳和裸鼠（源自nu/nu的瑞士小鼠）的不同器官中提取并纯化杆菌。总共对15个样本进行了重复检测，并将结果与代表34个物种的147株可培养分枝杆菌的检测结果进行比较。无论其来源或活力如何，所有样本中的麻风分枝杆菌菌株经BstEII酶切后均一致表现为315和135 bp的两个片段，经HaeIII酶切后表现为265和130 bp的两个片段。PRA是一种相对简单的方法，能够在种水平上对麻风分枝杆菌进行确定性鉴定。
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