da Silva Rocha A, Werneck Barreto A M, Dias Campos C E, Villas-Bôas da Silva M, Fonseca L, Saad M H, Degrave W M, Suffys P N
Biochemistry and Molecular Biology Departmentand, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
J Clin Microbiol. 2002 Nov;40(11):4191-6. doi: 10.1128/JCM.40.11.4191-4196.2002.
Human isolates of Mycobacterium collected in 16 different states of Brazil were submitted to PCR-restriction analysis (PRA) of a 439-bp fragment of the hsp65 gene with HaeIII and BstEII. Fourteen allelic variants not described in clinical isolates so far were observed among 36 (10%) of 356 Brazilian strains, including a new pattern for Mycobacterium scrofulaceum, M. intracellulare, and M. flavescens, two new patterns for M. fortuitum, three new patterns each for M. gordonae and M. terrae, and one new pattern for M. avium complex-like strains. Two unidentified strains each also presented a new pattern, strongly suggesting that Mycobacterium genotypes are distributed biogeographically. The PRA procedure was also performed with 43 reference isolates belonging to 34 species, adding a further six new patterns to the identification algorithm. A database containing the normalized restriction patterns of both enzymes was constructed. Patterns available on the Internet can be introduced into this database, which will make possible the comparison of genotypes from isolates from different parts of the world.
从巴西16个不同州收集的人源分枝杆菌菌株，采用HaeIII和BstEII对hsp65基因的439 bp片段进行PCR-限制性分析（PRA）。在356株巴西菌株中的36株（10%）中观察到14种迄今在临床分离株中未描述的等位基因变体，包括瘰疬分枝杆菌、胞内分枝杆菌和微黄分枝杆菌的一种新模式，偶然分枝杆菌的两种新模式，戈登分枝杆菌和土地分枝杆菌各三种新模式，以及鸟分枝杆菌复合群样菌株的一种新模式。另外有两株未鉴定菌株也呈现出一种新模式，强烈提示分枝杆菌基因型按生物地理学分布。还对属于34个种的43株参考菌株进行了PRA程序，在鉴定算法中又增加了六种新模式。构建了一个包含两种酶标准化限制性图谱的数据库。互联网上可用的图谱可导入该数据库，这将使得来自世界不同地区分离株的基因型比较成为可能。
