Pirnazar P, Wolinsky L, Nachnani S, Haake S, Pilloni A, Bernard G W
Section of Oral Biology, UCLA School of Dentistry, Los Angeles, CA 90024, USA.
J Periodontol. 1999 Apr;70(4):370-4. doi: 10.1902/jop.1999.70.4.370.
This investigation is one of a series of projects seeking to ascertain whether hyaluronic acid (HA) is therapeutically effective in tissue regeneration procedures. The rationale for these investigations is to test the hypothesis that HA can serve as a bioabsorbable carrier for other substrates as well as itself actively promote the regeneration of tissue.
In this paper, we report on the bacteriostatic and bactericidal properties of 3 molecular weight formulations of recombinant HA (low, 141 kD; medium, 757 kD; and high, 1,300 kD) on selected oral and non-oral microorganisms in the planktonic phase. Three concentrations of each HA formulation were screened, 0.5, 1.0, and 2.0 mg/ml, using a standard broth culture assay.
Recombinant HA exerted varied bacteriostatic effects on all the bacterial strains tested depending on its molecular weight (MW) and concentration. The high concentrations of the medium MW HA had the greatest bacteriostatic effect, particularly on the Actinobacillus actinomycetemcomitans, Prevotella oris, Staphylococcus aureus, and Propionibacterium acnes strains. The 1.0 mg/ml concentration of high MW HA had the greatest overall bacteriostatic effect, inhibiting the growth of all 6 bacterial strains tested. Among the bacterial strains studied, HA was found to have no bactericidal effects, regardless of concentration or molecular weight.
The results of this study suggest that HA in the MW range of 1,300 kD may prove beneficial in minimizing bacterial contamination of surgical wounds when used in guided tissue regeneration surgery.
本研究是一系列旨在确定透明质酸（HA）在组织再生过程中是否具有治疗效果的项目之一。这些研究的基本原理是检验以下假设：HA既可以作为其他底物的生物可吸收载体，又能够自身积极促进组织再生。
在本文中，我们报告了重组HA的三种分子量制剂（低分子量，141 kD；中等分子量，757 kD；高分子量，1300 kD）对浮游状态下选定的口腔和非口腔微生物的抑菌和杀菌特性。使用标准肉汤培养试验筛选了每种HA制剂的三种浓度，即0.5、1.0和2.0 mg/ml。
重组HA根据其分子量（MW）和浓度对所有测试细菌菌株产生不同的抑菌作用。中等分子量HA的高浓度具有最大的抑菌作用，特别是对伴放线放线杆菌、口腔普雷沃菌、金黄色葡萄球菌和痤疮丙酸杆菌菌株。高分子量HA的1.0 mg/ml浓度具有最大的总体抑菌作用，抑制了所有六种测试细菌菌株的生长。在所研究的细菌菌株中，无论浓度或分子量如何，HA均未发现有杀菌作用。
本研究结果表明，分子量在1300 kD范围内的HA在引导组织再生手术中使用时，可能有助于最大限度地减少手术伤口的细菌污染。
