Laywell E D, Kukekov V G, Steindler D A
Department of Anatomy and Neurobiology, University of Tennessee at Memphis, 855 Monroe Avenue, Memphis, Tennessee, 38163, USA.
Exp Neurol. 1999 Apr;156(2):430-3. doi: 10.1006/exnr.1999.7029.
The adult mammalian CNS harbors a population of multipotent stem/progenitor cells that can be induced to grow as proliferative neurospheres in vitro. We demonstrate here that neurosphere-generating cells can be isolated from adult mouse spinal cord and forebrain subependymal zone after postmortem intervals of up to 140 h, when kept at 4 degrees C, and up to 30 h when kept at room temperature. Although there was an inverse relationship between postmortem interval and the number of neurospheres generated, neurospheres derived under these conditions were proliferative and could give rise to both neurons and glia.
