Rennick B, Acara M, Hysert P, Mookerjee B
Kidney Int. 1976 Oct;10(4):329-35. doi: 10.1038/ki.1976.116.
Endogenous concentrations of free choline in plasma were measured in azotemic subjects receiving repetitive hemodialysis and excretion of free choline into the dialysate was determined. Chemical choline in plasma and dialysate was measured by adding choline kinase and measuring the production of radiolabelled phosphorycholine in the presence of radiolabelled adenosine triphosphate (ATP). Mean free choline concentration in plasma of azotemic subjects receiving hemodialysis was found to be 37 muM, which is about twice that of normal persons. The total excretion of choline into the dialysate during 360 min averaged 730 mumoles +/- 69 (SEM). Levels of free choline in plasma fell during hemodialysis at two hours but recovered toward predialysis values at six hours. The return of plasma choline concentrations toward control values during dialysis suggests that a feedback mechanism exists which was activated rapidly to produce homeostasis of plasma choline concentrations. In these patients, the degree of peripheral neuropathy as judged by measurement of nerve conduction velocities showed a significant inverse correlation with levels of free choline in plasma.
对接受重复血液透析的氮质血症患者血浆中游离胆碱的内源性浓度进行了测定，并确定了游离胆碱向透析液中的排泄情况。通过添加胆碱激酶并在存在放射性标记的三磷酸腺苷（ATP）的情况下测量放射性标记的磷酸胆碱的生成，来测定血浆和透析液中的化学胆碱。接受血液透析的氮质血症患者血浆中游离胆碱的平均浓度为37μM，约为正常人的两倍。在360分钟内胆碱向透析液中的总排泄量平均为730微摩尔±69（标准误）。血液透析两小时期间血浆中游离胆碱水平下降，但六小时时恢复到透析前值。透析期间血浆胆碱浓度恢复到对照值表明存在一种反馈机制，该机制被迅速激活以实现血浆胆碱浓度的稳态。在这些患者中，通过测量神经传导速度判断的周围神经病变程度与血浆中游离胆碱水平呈显著负相关。
