VITOTOX 细菌遗传毒性和毒性试验用于化学品的快速筛选。

VITOTOX bacterial genotoxicity and toxicity test for the rapid screening of chemicals.

作者信息

Verschaeve L, Van Gompel J, Thilemans L, Regniers L, Vanparys P, van der Lelie D

机构信息

Division of Environmental Toxicology, Flemish Institute for Technological Research, Mol, Belgium.

出版信息

Environ Mol Mutagen. 1999;33(3):240-8.

PMID:10334626

Abstract

The VITOTOX test is a new bacterial genotoxicity test that was previously shown to be very rapid and sensitive. Initially only one Salmonella typhimurium strain (TA104 recN2-4) was used in the test. In this paper we introduce a second strain (TA104pr1) that can be used as an internal control to further enhance the reliability of the test. We demonstrate the usefulness of this pr1 strain in genotoxicity and toxicity testing. We also report on the results of a study where the VITOTOX test was performed on newly synthesized pharmaceutical compounds, or intermediate products in the synthesis of drug candidates. We demonstrate that the test gives identical results when performed independently in two different laboratories and that it correlates well with either the Ames test or SOS chromotest.

摘要

VITOTOX试验是一种新型细菌遗传毒性试验，此前已证明该试验速度非常快且灵敏度高。最初该试验仅使用一种鼠伤寒沙门氏菌菌株（TA104 recN2 - 4）。在本文中，我们引入了第二种菌株（TA104pr1），它可用作内部对照，以进一步提高试验的可靠性。我们证明了这种pr1菌株在遗传毒性和毒性测试中的有用性。我们还报告了一项研究结果，该研究对新合成的药物化合物或候选药物合成中的中间产物进行了VITOTOX试验。我们证明，在两个不同实验室独立进行该试验时，会得到相同的结果，并且它与艾姆斯试验或SOS显色试验相关性良好。