精子染色体异常在复发性流产中的影响。
Implications of sperm chromosome abnormalities in recurrent miscarriage.
作者信息
Rubio C, Simón C, Blanco J, Vidal F, Mínguez Y, Egozcue J, Crespo J, Remohí J, Pellicer A
机构信息
Instituto Valenciano de Infertilidad, Valencia, Spain.
出版信息
J Assist Reprod Genet. 1999 May;16(5):253-8. doi: 10.1023/a:1020315529090.
PURPOSE
Our purpose was to assess the existence of sperm chromosome abnormalities in recurrent pregnancy loss in an assisted reproduction program.
METHODS
In this prospective study, 12 sperm samples from couples undergoing in vitro fertilization with two or more first-trimester spontaneous abortions were analyzed. Diploidy and disomy in decondensed sperm nuclei were assessed for chromosomes 13, 18, 21, X, and Y using two- and three-color fluorescence in situ hybridization.
RESULTS
Sex chromosome disomy in sperm samples from recurrent abortion couples was significantly increased compared to that from internal controls (0.84% vs 0.37%). In a subpopulation of seven couples who underwent oocyte donation, mean frequencies for sex chromosome disomy (1%) were even higher and diploidy (0.43%) was also significantly increased.
CONCLUSIONS
These results suggest an implication of sperm chromosome abnormalities in some cases of recurrent pregnancy loss.
目的
我们的目的是评估辅助生殖项目中复发性流产患者精子染色体异常的存在情况。
方法
在这项前瞻性研究中，对12例经历过两次或更多次孕早期自然流产且正在接受体外受精的夫妇的精子样本进行了分析。使用双色和三色荧光原位杂交技术，对去浓缩精子核中的13、18、21、X和Y染色体的二倍体和双体情况进行了评估。
结果
与内部对照相比，复发性流产夫妇精子样本中的性染色体双体显著增加（0.84%对0.37%）。在接受卵母细胞捐赠的7对夫妇亚组中，性染色体双体的平均频率（1%）甚至更高，并且二倍体（0.43%）也显著增加。
结论
这些结果表明精子染色体异常在某些复发性流产病例中具有一定作用。
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