Bartholdy C, Nansen A, Marker O, Thomsen A R
Institute of Medical Microbiology and Immunology, Panum Institute, Copenhagen, Denmark.
Clin Exp Immunol. 1999 May;116(2):299-306. doi: 10.1046/j.1365-2249.1999.00903.x.
The role of soluble receptors for TNF-alpha (sTNF-Rs) as markers of virus-induced host responses was studied by the use of murine model infections. A marked elevation in serum levels of sTNF-R75, but not sTNF-R55, was found 1 day after infection with vesicular stomatitis virus (VSV). In mice infected with lymphocytic choriomeningitis virus (LCMV), an early increase was also revealed, but peak levels of sTNF-R75 were observed later temporally related to maximal T cell-mediated anti-viral activity. Analysing different well characterized knockout mice, it was found that elevated release of sTNF-R75 into serum early after VSV infection was independent of T cells, whereas interferon (IFN)-alpha/beta seemed to be a major mediator. In contrast, increased release of sTNF-R75 into serum 8 days post-LCMV infection was mediated via T cells but independently of both CD40 ligand and IFN-gamma. A simple correlation between release of sTNF-Rs in vivo and macrophage activation in vitro was not present. These findings indicate that sTNF-R75 is indeed a sensitive marker of both innate and specific cell-mediated host reactivity during viral infection, but it is not correlated to a single immunological parameter.
通过使用小鼠模型感染，研究了可溶性肿瘤坏死因子-α受体（sTNF-Rs）作为病毒诱导宿主反应标志物的作用。感染水疱性口炎病毒（VSV）1天后，发现血清中sTNF-R75水平显著升高，但sTNF-R55未升高。在感染淋巴细胞性脉络丛脑膜炎病毒（LCMV）的小鼠中，也观察到早期升高，但sTNF-R75的峰值水平在时间上与最大T细胞介导的抗病毒活性相关。分析不同特征明确的基因敲除小鼠发现，VSV感染后早期血清中sTNF-R75释放增加与T细胞无关，而干扰素（IFN）-α/β似乎是主要介质。相反，LCMV感染8天后血清中sTNF-R75释放增加是由T细胞介导的，但与CD40配体和IFN-γ均无关。体内sTNF-Rs释放与体外巨噬细胞活化之间不存在简单的相关性。这些发现表明，sTNF-R75确实是病毒感染期间先天性和特异性细胞介导的宿主反应性的敏感标志物，但它与单一免疫参数无关。
