Lohmeyer J, Friedrich J, Grimminger F, Maus U, Tenter R, Morr H, Velcovsky H G, Seeger W, Rosseau S
Department of Internal Medicine, Justus-Liebig-University, Giessen, Germany.
Clin Exp Immunol. 1999 May;116(2):340-6. doi: 10.1046/j.1365-2249.1999.00894.x.
The expression of alphaEbeta7 integrin has been related to the selective retention of lymphocytes in mucosal tissues of gut, urogenital tract and lung. To identify potential disease-associated alphaEbeta7 expression patterns on cells accounting for lymphocytic alveolitis in interstitial lung disease (ILD), alphaE expression on CD4+ and CD8+ T cell subsets was evaluated by dual-colour flow cytometry in peripheral blood and bronchoalveolar lavage fluid (BALF) of patients with idiopathic pulmonary fibrosis (IPF; n = 18), hypersensitivity pneumonitis (HP; n = 20) and sarcoidosis (n = 44) in comparison with healthy controls (n = 15). In both healthy individuals and all patient groups the proportion of alphaE-bearing T cells in peripheral blood was < 2%, whereas the vast majority of alveolar CD8+ T cells consistently co-expressed alphaE. Absolute alveolar CD8+alphaE+ cell numbers/ml were up to 30-fold increased in HP patients. Proportions of alphaE-bearing CD4+ cells in BALF were significantly elevated in IPF (74.0 +/- 2.7%) and HP (70.0 +/- 2.4%) compared with normals (30.0 +/- 1.8%) (mean +/- s.e.m.; P < 0.01). In sarcoidosis, the alphaE expression on BALF CD4+ cells displayed subgroup dependency: proportions significantly lower than normal were noted in chest radiographic stage I (14.3 +/- 1.5%), but increased proportions in stages II (50.0 +/- 3.8%) and III (64.0 +/- 4.8%). Correlations between common markers of T cell activation or BALF transforming growth factor-beta (TGF-beta ) bioactivity and alphaE expression were not noted. We conclude that the vast majority of alveolar CD8+ T cells consistently express alphaEbeta7 and that distinct patterns of alphaEbeta7 expression on alveolar CD4+ lymphocytes in sarcoidosis are related to the diverse manifestations of the sarcoid inflammatory process in the lung.
αEβ7整合素的表达与淋巴细胞在肠道、泌尿生殖道和肺的黏膜组织中的选择性滞留有关。为了确定间质性肺疾病（ILD）中导致淋巴细胞性肺泡炎的细胞上潜在的疾病相关αEβ7表达模式，通过双色流式细胞术评估了特发性肺纤维化（IPF；n = 18）、过敏性肺炎（HP；n = 20）和结节病（n = 44）患者外周血和支气管肺泡灌洗液（BALF）中CD4 +和CD8 + T细胞亚群上的αE表达，并与健康对照（n = 15）进行比较。在健康个体和所有患者组中，外周血中携带αE的T细胞比例均<2%，而绝大多数肺泡CD8 + T细胞始终共表达αE。HP患者每毫升肺泡CD8 +αE +细胞的绝对数量增加了30倍。与正常人（30.0 +/- 1.8%）相比，IPF（74.0 +/- 2.7%）和HP（70.0 +/- 2.）患者BALF中携带αE的CD4 +细胞比例显著升高（平均值 +/- 标准误；P < 0.01）。在结节病中，BALF CD4 +细胞上的αE表达表现出亚组依赖性：胸部X线分期I期（14.3 +/- 1.5%）的比例显著低于正常，但II期（50.0 +/- 3.8%）和III期（64.0 +/- 4.8%）的比例增加。未发现T细胞活化的常见标志物或BALF转化生长因子-β（TGF-β）生物活性与αE表达之间的相关性。我们得出结论，绝大多数肺泡CD8 + T细胞始终表达αEβ7，并且结节病中肺泡CD4 +淋巴细胞上αEβ7表达的不同模式与肺部结节病炎症过程的不同表现有关。
