Seidel K E, Stolte M, Lehn N, Bauer J
Technische Universität München, Freising-Weihenstephan, Germany.
Zentralbl Veterinarmed B. 1999 Apr;46(3):181-8. doi: 10.1046/j.1439-0450.1999.00219.x.
Serum samples from 61 dogs and 49 cats were screened for circulating antibodies against Helicobacter felis by an enzyme-linked immunosorbent assay (ELISA) using sonicated bacteria as an antigen. To improve the specificity of the ELISA, sera were absorbed with Campylobacter jejuni subsp. jejuni H. pylori as well as H. felis. Sera from 26 dogs (43%) and 19 cats (39%) revealed clear positive absorbance readings determined as an optical density (OD) that was statistically significant above the OD mean value [P < 0.025 (one-tailed); log10]. The absorbance pattern of ELISA-positive sera corresponded to results obtained with bovine and human reference sera. Furthermore, a correlation between the immune response and results from histopathological examination of gastric specimens from 22 dogs was demonstrated. It could be shown that antibodies against H. felis in sera of cats and dogs can easily be detected using an ELISA. The diagnostic value of this test must be evaluated in further investigations.
采用超声破碎细菌作为抗原，通过酶联免疫吸附测定（ELISA）对61只犬和49只猫的血清样本进行检测，以筛查抗猫螺杆菌的循环抗体。为提高ELISA的特异性，血清用空肠弯曲菌空肠亚种、幽门螺杆菌以及猫螺杆菌进行吸收处理。26只犬（43%）和19只猫（39%）的血清显示出明确的阳性吸光度读数，以光密度（OD）表示，该读数在统计学上显著高于OD平均值[P < 0.025（单尾）；log10]。ELISA阳性血清的吸光度模式与牛和人参考血清的结果一致。此外，还证明了22只犬的免疫反应与胃标本组织病理学检查结果之间存在相关性。结果表明，使用ELISA可以轻松检测猫和犬血清中抗猫螺杆菌的抗体。该检测的诊断价值必须在进一步的研究中进行评估。
