Hwang Cheol-Yong, Han Hong-Ryul, Youn Hwa-Young
Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Republic of Korea.
J Vet Sci. 2002 Jun;3(2):123-33.
This study was carried out to evaluate the prevalence and clinical characterizations of gastric Helicobacter spp. infection of dogs and cats in Korea. The prevalence of Helicobacter spp. infection of dogs and cats determined by urease test was 78.4% and 64%, respectively, although Helicobacter genus-specific PCR assay showed that it was 82.3% and 84%. Urease mapping results based on urease test showed that total positive rate of tested tissues from clinically abnormal dogs was significantly higher than that from clinically normal dogs (p=0.0018; Odds ratio = 6.118; 95% Confidence Interval = 1.96-19.103). These findings were consistent with the results of Helicobacter genus-specific PCR assay which showed that positive rate of the fundus (100%) and the antrum (100%) of clinically abnormal dogs was significantly higher than that of same gastric regions of clinically normal dogs (77.5 and 67.5% respectively). In comparison of gastric regions between clinically normal dogs and abnormal dogs, positive rate of urease test for the fundus (100%) and body (90.9%) in clinically abnormal dogs was significantly higher than that of abnormal dogs (72.5% and 57.5% respectively; p<0.05). The results of urease mapping in dogs and cats also indicated that Helicobacter colonization in the fundus was more dense compared with the density in the body and antrum. In Helicobacter species-specific PCR assay for dogs, 32 of 42 fundic tissues (76.2%) were positive for H. heilmannii and two (4.8%) were positive for H. felis. In cats, 18 of 21 fundic tissues (85.7%) were positive for H. heilmannii and 2 (9.5%) were positive for H. felis. Gastritis scores of fundic tissues from clinically abnormal infected dogs were similar to that from noninfected dogs and evidence of upregulation of IL-1beta, IL-8, and TNF-alpha mRNA was not detected in gastric fundic tissues from clinically abnormal infected dogs. This study suggested that Helicobacter spp. infection in domestic dogs including private owned pet dogs and cats is highly prevalent usually with no clinical sign but high density of colonization can be related to gastrointestinal signs
本研究旨在评估韩国犬猫胃内幽门螺杆菌属感染的患病率及临床特征。通过尿素酶试验确定的犬猫幽门螺杆菌属感染患病率分别为78.4%和64%，而幽门螺杆菌属特异性PCR检测显示分别为82.3%和84%。基于尿素酶试验的尿素酶定位结果显示，临床异常犬的受试组织总阳性率显著高于临床正常犬（p = 0.0018；优势比 = 6.118；95%置信区间 = 1.96 - 19.103）。这些结果与幽门螺杆菌属特异性PCR检测结果一致，该检测显示临床异常犬胃底（100%）和胃窦（100%）的阳性率显著高于临床正常犬相同胃区域（分别为77.5%和67.5%）。比较临床正常犬和异常犬的胃区域，临床异常犬胃底（100%）和胃体（90.9%）的尿素酶试验阳性率显著高于异常犬（分别为72.5%和57.5%；p < 0.05）。犬猫的尿素酶定位结果还表明，与胃体和胃窦相比，胃底的幽门螺杆菌定植密度更高。在犬的幽门螺杆菌种特异性PCR检测中，42个胃底组织中有32个（76.2%）海氏螺杆菌阳性，2个（4.8%）猫螺杆菌阳性。在猫中，21个胃底组织中有18个（85.7%）海氏螺杆菌阳性，2个（9.5%）猫螺杆菌阳性。临床异常感染犬胃底组织的胃炎评分与未感染犬相似，且在临床异常感染犬的胃底组织中未检测到IL-1β、IL-8和TNF-α mRNA上调的证据。本研究表明，包括私人宠物犬和猫在内的家犬幽门螺杆菌属感染非常普遍，通常无临床症状，但高定植密度可能与胃肠道症状有关
