Strauss-Ayali D, Scanziani E, Deng D, Simpson K W
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Vet Microbiol. 2001 Apr 2;79(3):253-65. doi: 10.1016/s0378-1135(00)00360-6.
The principal aims of this study were to evaluate the humoral immune response (IgG) of cats with gastric Helicobacter spp. infection, and to determine the prevalence of different types of Helicobacter spp. in the stomachs of cats. The Helicobacter infection status of 45 cats (12 healthy spay/neuter cats, 9 sick cats, 24 colony cats) was determined by evaluating endoscopic gastric biopsies for urease activity, presence of Helicobacter-like organisms (HLO) on histopathology, and genus and species-specific PCR. Serum samples were evaluated with a kinetic enzyme linked immunosorbent assay (ELISA) utilizing the high molecular cell-associated protein (HM-CAP) fraction of H. felis ATCC 49179.Seventeen of 45 cats were infected with Helicobacter spp.: "H. heilmannii" 9/17, H. felis 4/17, mixed "H. heilmannii" and H. felis 3/17, unclassified-Helicobacter spp. 7/17. H. pylori was not detected in any cat. Kinetic ELISA results were significantly higher for infected cats, than for uninfected cats. Cats infected with different Helicobacter spp. showed similar distribution of OD/min values. There were no effects of age or clinical signs on the results of kinetic ELISA. No correlation between colonization density and seroconversion was observed. There were statistically significant, but weak correlations between the degree of seroconversion and the degree of inflammation, and the number of lymphoid follicles. Infected cats had more severe inflammation in the pylorus and fundus than uninfected cats. Infected sick cats had a higher degree of pyloric, but not fundic inflammation, than healthy infected cats and uninfected sick cats. The results indicate that naturally acquired infection with gastric Helicobacter spp. is associated with seroconversion (IgG) in cats. The similar ELISA values in cats infected with a variety of Helicobacter spp. suggests substantial antigenic homology between different Helicobacter spp. The higher degree of inflammation in infected than uninfected cats, supports a role for Helicobacter as a cause of gastritis in cats.
本研究的主要目的是评估感染胃幽门螺杆菌属的猫的体液免疫反应（IgG），并确定猫胃中不同类型幽门螺杆菌属的流行情况。通过评估内镜下胃活检的尿素酶活性、组织病理学上幽门螺杆菌样生物体（HLO）的存在以及属和种特异性PCR，确定了45只猫（12只健康绝育猫、9只病猫、24只群体饲养猫）的幽门螺杆菌感染状况。血清样本采用动力学酶联免疫吸附测定（ELISA）进行评估，该方法利用了猫螺杆菌ATCC 49179的高分子细胞相关蛋白（HM-CAP）部分。45只猫中有17只感染了幽门螺杆菌属：“海尔曼幽门螺杆菌”9/17，猫螺杆菌4/17，“海尔曼幽门螺杆菌”和猫螺杆菌混合感染3/17，未分类的幽门螺杆菌属7/17。在任何猫中均未检测到幽门螺杆菌。感染猫的动力学ELISA结果显著高于未感染猫。感染不同幽门螺杆菌属的猫的OD/分钟值分布相似。年龄或临床症状对动力学ELISA结果没有影响。未观察到定植密度与血清转化之间的相关性。血清转化程度与炎症程度以及淋巴滤泡数量之间存在统计学上显著但较弱的相关性。感染猫的幽门和胃底炎症比未感染猫更严重。感染的病猫幽门炎症程度高于健康感染猫和未感染病猫，但胃底炎症程度并非如此。结果表明，猫自然获得的胃幽门螺杆菌属感染与血清转化（IgG）有关。感染多种幽门螺杆菌属的猫的ELISA值相似，表明不同幽门螺杆菌属之间存在大量抗原同源性。感染猫比未感染猫炎症程度更高，这支持了幽门螺杆菌作为猫胃炎病因的作用。
