Everest P, Ketley J, Hardy S, Douce G, Khan S, Shea J, Holden D, Maskell D, Dougan G
Department of Biochemistry, Imperial College of Science, Technology and Medicine, London, United Kingdom.
Infect Immun. 1999 Jun;67(6):2815-21. doi: 10.1128/IAI.67.6.2815-2821.1999.
Salmonella typhimurium strains harboring independent, defined mutations in aroA, invA, ssrA, or msbB were assessed for their ability to induce fluid accumulation, tissue damage, and local inflammation in rabbit ileal loops. Three wild-type strains of S. typhimurium, TML, HWSH, and SL1344, and two mutant strains, S. typhimurium SL1344 ssrA and S. typhimurium SL1344 msbB, consistently induced fluid accumulation in the lumen of loops and inflammation of loop-associated tissues. In contrast, three different S. typhimurium aroA strains and an invA mutant of SL1344 did not induce significant fluid accumulation in the rabbit ileal loops. However, the S. typhimurium aroA strains did induce an inflammatory infiltrate and some local villus-associated damage, but the invA mutant did not. Histologically, wild-type S. typhimurium, S. typhimurium SL1344 ssrA, and S. typhimurium SL1344 msbB demonstrated more severe effects on villus architecture than S. typhimurium aroA strains, whereas S. typhimurium invA-infected loops showed no detectable damage. This suggests that villus damage most likely contributes to fluid accumulation within the loop.
对携带aroA、invA、ssrA或msbB独立、明确突变的鼠伤寒沙门氏菌菌株，评估其在兔回肠袢中诱导液体蓄积、组织损伤和局部炎症的能力。三株野生型鼠伤寒沙门氏菌菌株TML、HWSH和SL1344，以及两株突变菌株鼠伤寒沙门氏菌SL1344 ssrA和鼠伤寒沙门氏菌SL1344 msbB，始终能诱导袢腔内液体蓄积及袢相关组织炎症。相比之下，三株不同的鼠伤寒沙门氏菌aroA菌株和SL1344的invA突变体在兔回肠袢中未诱导出显著的液体蓄积。然而，鼠伤寒沙门氏菌aroA菌株确实诱导了炎性浸润和一些局部绒毛相关损伤，但invA突变体没有。组织学上，野生型鼠伤寒沙门氏菌、鼠伤寒沙门氏菌SL1344 ssrA和鼠伤寒沙门氏菌SL1344 msbB对绒毛结构的影响比鼠伤寒沙门氏菌aroA菌株更严重，而感染鼠伤寒沙门氏菌invA的袢未显示出可检测到的损伤。这表明绒毛损伤很可能是导致袢内液体蓄积的原因。
