Marshall D G, Hynes S O, Coleman D C, O'Morain C A, Smyth C J, Moran A P
Department of Microbiology, Moyne Institute for Preventive Medicine, Trinity College, Dublin, Ireland.
FEMS Immunol Med Microbiol. 1999 May;24(1):79-90. doi: 10.1111/j.1574-695X.1999.tb01268.x.
The cagA gene, vacA gene, CagA (cytotoxin-associated gene A product) and VacA (vacuolating cytotoxin) status of a collection of Helicobacter pylori isolates from the geographically distinct Irish population was determined, the potential association of these traits with Lewis (Le) antigen expression was assessed, and the relationship between these bacterial properties and the pathology associated with H. pylori infection was evaluated. Of the 57 isolates, a higher proportion from ulcer than from non-ulcer patients expressed VacA (71% vs. 53%). H. pylori isolates which were cagA-positive were no more significantly associated with peptic ulcers than non-ulcer disease (71% vs. 67%, P = 0.775), nor were CagA-positive isolates (57% vs. 50%, P = 0.783), but 80% of the isolates from duodenal ulcer patients were cagA-positive. Thirty-seven of the 57 isolates were tested for Le antigen expression. No statistically significant relationship (P > 0.05) was found between the occurrence and level of expression of Le(x) or Le(y) and cagA, vacA, or VacA status. This lack of an association in the Irish H. pylori isolates contrasts with that previously reported for predominantly North American isolates, and may be attributable to the adaptation of H. pylori strains with differing attributes to different human populations.
对从地理位置不同的爱尔兰人群中分离出的一批幽门螺杆菌菌株的cagA基因、vacA基因、CagA（细胞毒素相关基因A产物）和VacA（空泡毒素）状态进行了测定，评估了这些特征与Lewis（Le）抗原表达的潜在关联，并评价了这些细菌特性与幽门螺杆菌感染相关病理学之间的关系。在57株分离菌株中，来自溃疡患者的菌株中表达VacA的比例高于非溃疡患者（71%对53%）。cagA阳性的幽门螺杆菌分离株与消化性溃疡的相关性并不比与非溃疡疾病的相关性更显著（71%对67%，P = 0.775），CagA阳性分离株也是如此（57%对50%，P = 0.783），但十二指肠溃疡患者分离株中有80%为cagA阳性。对57株分离菌株中的37株进行了Le抗原表达检测。未发现Le(x)或Le(y)的出现及表达水平与cagA、vacA或VacA状态之间存在统计学显著关系（P > 0.05）。爱尔兰幽门螺杆菌分离株中缺乏这种关联，这与先前主要针对北美分离株报道的情况形成对比，这可能归因于具有不同属性的幽门螺杆菌菌株对不同人群的适应性。
