Talebi Bezmin Abadi Amin, Mohabbati Mobarez Ashraf
Department of Medical Microbiology, Utrecht University, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands.
Department of Bacteriology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran.
J Pathog. 2014;2014:842469. doi: 10.1155/2014/842469. Epub 2014 Feb 10.
Helicobacter pylori plays an important role in the pathogenesis of chronic gastritis, peptic ulceration, and noncardia gastric cancer. Several putative virulence factors for H. pylori have been identified including vacA, babA, and iceA. HopQ is one of the outer membrane proteins involved in bacterial adherence to gastric mucosa and has been suggested to also play a role in the virulence of H. pylori. Due to the substantial geographic differences in the prevalence of H. pylori virulence factors reported, the main purpose of the current study was to investigate the association between different H. pylori virulence hopQ alleles (types I and II) and patients with gastroduodenal disorders. The presence of H. pylori and hopQ alleles in gastric biopsy specimens was identified by specific PCR assays. H. pylori type II hopQ was found to be significantly associated with gastric cancer patients (odds ratio: 3.47, 95% CI: 1.56-5.89). Information about the prevalence of H. pylori hopQ type II can be used for determining the high-risk diseases type which is actually colonized by H. pylori hopQ type II positive strains. The presence of H. pylori hopQ type II should be investigated in different geographical regions as confirmatory findings may provide a definite biomarker attributed to the pathogenesis of certain severe digestive diseases.
幽门螺杆菌在慢性胃炎、消化性溃疡和非贲门胃癌的发病机制中起重要作用。已鉴定出几种幽门螺杆菌的假定毒力因子，包括vacA、babA和iceA。HopQ是参与细菌黏附胃黏膜的外膜蛋白之一，也被认为在幽门螺杆菌的毒力中起作用。由于报道的幽门螺杆菌毒力因子流行率存在显著地理差异，本研究的主要目的是调查不同幽门螺杆菌毒力hopQ等位基因（I型和II型）与胃十二指肠疾病患者之间的关联。通过特异性PCR检测确定胃活检标本中幽门螺杆菌和hopQ等位基因的存在。发现幽门螺杆菌II型hopQ与胃癌患者显著相关（比值比：3.47，95%可信区间：1.56 - 5.89）。幽门螺杆菌II型hopQ流行率的信息可用于确定实际被幽门螺杆菌II型阳性菌株定植的高危疾病类型。应在不同地理区域调查幽门螺杆菌II型hopQ的存在情况，因为确认性结果可能提供一种明确的生物标志物，归因于某些严重消化系统疾病的发病机制。
