Suau P, Kneale G G, Braddock G W, Baldwin J P, Bradbury E M
Nucleic Acids Res. 1977 Nov;4(11):3769-86. doi: 10.1093/nar/4.11.3769.
Neutron scattering studies have been applied to chromatin core particles in solution, using the contrast variation technique. On the basis of the contrast dependance of the radius of gyration and the radial distribution function it is shown that the core particle consists of a core containing most of the histone around which is wound the DNA helix,following a path with a mean radius of 4.5 nm,in association with a small proportion of the histones. Separation of the shape from the internal structure, followed by model calculations shows that the overall shape of the particle is that of a flat cylinder with dimensions ca. 11x11x6 nm. Further details of the precise folding of the DNA cannot be deduced from the data, but detailed model calculations support concurrent results from crystallographic studies(25).Images
利用对比变化技术，中子散射研究已应用于溶液中的染色质核心颗粒。基于回转半径和径向分布函数的对比依赖性，结果表明核心颗粒由一个包含大部分组蛋白的核心组成，DNA螺旋围绕该核心缠绕，其路径的平均半径为4.5纳米，并与一小部分组蛋白相结合。将形状与内部结构分离，随后进行模型计算，结果表明颗粒的整体形状为扁平圆柱体，尺寸约为11×11×6纳米。从这些数据中无法推断出DNA精确折叠的更多细节，但详细的模型计算支持了晶体学研究的同期结果(25)。图像
