番茄早疫病菌中的多种环氧化物水解酶及其与培养基成分和寄主专化性毒素产生的关系。
Multiple epoxide hydrolases in Alternaria alternata f. sp. lycopersici and their relationship to medium composition and host-specific toxin production.
作者信息
Morisseau C, Ward B L, Gilchrist D G, Hammock B D
机构信息
Department of Entomology, University of California, Davis, California 95616, USA.
出版信息
Appl Environ Microbiol. 1999 Jun;65(6):2388-95. doi: 10.1128/AEM.65.6.2388-2395.1999.
Abstract
The production of Alternaria alternata f. sp. lycopersici host-specific toxins (AAL toxins) and epoxide hydrolase (EH) activity were studied during the growth of this plant-pathogenic fungus in stationary liquid cultures. Media containing pectin as the primary carbon source displayed peaks of EH activity at day 4 and at day 12. When pectin was replaced by glucose, there was a single peak of EH activity at day 6. Partial characterization of the EH activities suggests the presence of three biochemically distinguishable EH activities. Two of them have a molecular mass of 25 kDa and a pI of 4.9, while the other has a molecular mass of 20 kDa and a pI of 4.7. Each of the EH activities can be distinguished by substrate preference and sensitivity to inhibitors. The EH activities present at day 6 (glucose) or day 12 (pectin) are concomitant with AAL toxin production.
摘要
在静止液体培养条件下，研究了番茄链格孢菌专化型（Alternaria alternata f. sp. lycopersici）宿主特异性毒素（AAL毒素）的产生及环氧水解酶（EH）活性。以果胶作为主要碳源的培养基在第4天和第12天出现EH活性峰值。当果胶被葡萄糖取代时，在第6天出现单一的EH活性峰值。对EH活性的部分特性分析表明存在三种生化特性可区分的EH活性。其中两种的分子量为25 kDa，等电点为4.9，而另一种的分子量为20 kDa，等电点为4.7。每种EH活性可通过底物偏好性和对抑制剂的敏感性来区分。第6天（葡萄糖）或第12天（果胶）出现的EH活性与AAL毒素的产生同步。
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2
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6
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