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筛选产环氧化物水解酶的微生物用于脱氧雪腐镰刀菌烯醇的生物转化。

Screening of epoxide hydrolase producing microorganisms for biotransformation of deoxynivalenol.

机构信息

Institute for Phytomedicine, University of Hohenheim, Otto-Sander-Straße 5, D-70593, Stuttgart, simone

出版信息

Mycotoxin Res. 2005 Mar;21(1):71-3. doi: 10.1007/BF02954823.

Abstract

Deoxynivalenol (DON) transformation products from selected time course experiments were analyzed by thin-layer chromatography. With the strainAlternaria alternata f. sp.lycopersici AS27-3, one major metabolite of DON in ethyl acetate was observed. This unidentified metabolite was more polar than DON and has a Rf value of 0.71. Derivatization indicated that this metabolite was probably an unidentified trichothecene. Screening of 29 other microbial isolates (bacteria, yeast, filamentous fungi) for DON transformation did not result in any active organism.

摘要

采用薄层层析法分析了从选定的时间进程实验中得到的脱氧雪腐镰刀菌烯醇(DON)转化产物。用番茄早疫病菌 AS27-3 菌株,在乙酸乙酯中观察到 DON 的一种主要代谢产物。这种未鉴定的代谢物比 DON 更具极性,Rf 值为 0.71。衍生化表明,这种代谢物可能是一种未鉴定的三萜烯。对 29 种其他微生物分离物(细菌、酵母、丝状真菌)进行 DON 转化的筛选没有得到任何活性生物。

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