Esumi Y, Suzuki Y, Kimura K, Yoshihama M, Ichikawa T, Uramoto M
The Institute of Physical and Chemical Research (RIKEN), Saitama, Japan.
J Antibiot (Tokyo). 1999 Mar;52(3):281-7. doi: 10.7164/antibiotics.52.281.
The structures of the fatty acid components of various new liposidomycins were determined using tandem mass spectrometry; the negative FAB/MS/MS/MS technique for liposidomycins with a 3-methylglutaric acid moiety and the negative FAB/MS/MS technique for liposidomycins without a 3-methylglutaric acid moiey. This structural information was obtained by analysis of peaks due to charge-remote fragmentations for 3-hydroxycarboxylate anions or carboxylate anions which were generated from liposidomycin molecules in a mass spectrometer. The MS/MS/MS technique that can exclude the matrix-derived and/or interfering ions showed higher sensitivity than the MS/MS technique.
利用串联质谱法测定了各种新型脂霉素脂肪酸成分的结构；对于含有3-甲基戊二酸部分的脂霉素采用负离子快原子轰击串联质谱法（FAB/MS/MS/MS），对于不含3-甲基戊二酸部分的脂霉素采用负离子快原子轰击质谱法（FAB/MS/MS）。该结构信息是通过分析质谱仪中脂霉素分子产生的3-羟基羧酸根阴离子或羧酸根阴离子的电荷远程碎裂峰而获得的。能够排除基质衍生离子和/或干扰离子的串联质谱法（MS/MS/MS）比质谱法（MS/MS）灵敏度更高。
