Frankel C S, Ellingboe A H
Mol Gen Genet. 1976 Nov 17;148(3):225-31. doi: 10.1007/BF00332896.
Common-AB diploids with several heterozygous biochemical markers were mated with appropriately marked haploid strains of S. commune in an effort to obtain compatible, common-A, and common-B diploid progeny with biochemical markers identical to those of the common-AB parent. The spores from these crosses were germinated on minimal medium. Five compatible diploids, but no common-A or common-B diploids, marked as desired, were isolated by this method. Two possessed some dikaryotic cells and two had many dikaryotic cells. One of the latter was shown to have peculiar behaviour associated with one of its B mating-type factors.
具有几个杂合生化标记的普通AB二倍体与适当标记的香菇单倍体菌株进行交配，以获得与普通AB亲本具有相同生化标记的兼容的普通A和普通B二倍体后代。这些杂交产生的孢子在基本培养基上萌发。通过这种方法分离出了5个符合要求的兼容二倍体，但没有普通A或普通B二倍体。其中两个含有一些双核细胞，另外两个有许多双核细胞。后者中的一个被证明具有与其一个B交配型因子相关的特殊行为。
