Usen S O, Akinyinka O O, Akanni O A, Ajaiyeoba I A, Falade A G, Osinusi K, Akang E E
Department of Pediatrics, College of Medicine, University College Hospital, Ibadan, Nigeria.
Acta Cytol. 1999 May-Jun;43(3):416-21. doi: 10.1159/000331090.
To determine vitamin A status by conjunctival impression cytology with transfer (CIC-T) and assess its ability to predict low and deficient serum retinol concentrations.
CIC-T was performed on 128 healthy, well-nourished and 230 malnourished children aged under 6 years by a 3-5-second application of cellulose acetate paper to each bulbar conjunctiva followed by transfer of the adhered cells onto glass slides. The slides were stained with Alcian green 2GX, and smears were classified as normal, borderline normal, borderline abnormal and deficient. Corresponding serum retinol levels were determined in each subject.
The results showed that CIC-T is a simple procedure with a failure rate of 7.3% caused by tearing and agitation. The power of CIC-T to predict vitamin A status varied with both the CIC-T smear classification used and serum retinol concentration threshold. CIC-T smear classification as abnormal and normal appears to be the most robust and predictive of serum retinol, < 10 and > 10 < 20 micrograms/dL, respectively.
The simplicity, sensitivity and specificity of CIC-T suggest that this procedure is a good screening tool for epidemiologic survey of vitamin A status.
通过结膜印迹细胞学转移法（CIC-T）确定维生素A状态，并评估其预测血清视黄醇浓度低和缺乏的能力。
对128名健康、营养良好的6岁以下儿童和230名营养不良的儿童进行CIC-T，将醋酸纤维素纸在每个球结膜上贴3 - 5秒，然后将粘附的细胞转移到载玻片上。玻片用阿尔辛蓝2GX染色，涂片分为正常、临界正常、临界异常和缺乏。测定每个受试者相应的血清视黄醇水平。
结果表明，CIC-T是一个简单的操作，因流泪和躁动导致的失败率为7.3%。CIC-T预测维生素A状态的能力因所使用的CIC-T涂片分类和血清视黄醇浓度阈值而异。CIC-T涂片分类为异常和正常似乎对血清视黄醇分别<10和>10<20微克/分升的情况最具稳健性和预测性。
CIC-T的简单性、敏感性和特异性表明，该方法是维生素A状态流行病学调查的良好筛查工具。
