Astaldi G, Karanovic D, Scorza R, Karanovic J, Topuz U
Boll Ist Sieroter Milan. 1976;55(5):386-95.
It was previously reported that PHA-incubation of a pool of peripheral-blood lymphocytes before their i.v. injection into rats submitted to total body irradiation, stimulates spleen colony formation and growth. On the same model, the present study has investigated the effect of lymphocyte preincubation with supernates obtained in short term cultures from human lymphocytes prestimulated with PHA (lymphokines). The "in vitro" culture of rat lymphocytes in a medium supplemented with human lymphokines showed either a marked increase of lymphocyte survival rate, or significant stimulation of lymphocyte blastogenesis, but at an extent much lower than that caused by PHA, also because in this particular experiment the culture medium was supplemented with too little amount of lymphokines. These data support the interpretation that human lymphokines may act across the species barriers. Peripheral-blood rat lymphocytes prestimulated with human lymphokines, and then injected i.v. into rats previously submitted to total body irradiation, caused significant increase in the spleen weight, as well as formation of spleen colonies quite larger and with higher erythroid differentiation - though less numerous than in the controls (i.e., in the rats injected just with untreated lymphocytes). This behaviour may be due to the tendency to merge together of two or more colonies in a single giant colony, and supports the interpretation that immune-competent lymphocytes activated either with PHA or with lymphokines may stimulate spleen colony growth and erythroid differentiation, though at different extents.
先前有报道称，将一组外周血淋巴细胞在静脉注射到接受全身照射的大鼠体内之前进行PHA孵育，可刺激脾集落的形成和生长。在同一模型上，本研究调查了淋巴细胞与从经PHA预刺激的人淋巴细胞短期培养物中获得的上清液（淋巴因子）进行预孵育的效果。在补充了人淋巴因子的培养基中对大鼠淋巴细胞进行“体外”培养，结果显示淋巴细胞存活率显著提高，或淋巴细胞增殖明显受到刺激，但程度远低于PHA所引起的，这也是因为在这个特定实验中培养基中补充的淋巴因子量太少。这些数据支持了人淋巴因子可能跨越物种屏障发挥作用的解释。用人淋巴因子预刺激的外周血大鼠淋巴细胞，然后静脉注射到先前接受全身照射的大鼠体内，导致脾脏重量显著增加，以及形成相当大且具有更高红系分化的脾集落——尽管数量比对照组（即仅注射未处理淋巴细胞的大鼠）少。这种行为可能是由于两个或更多集落倾向于合并成一个巨大集落，并且支持了这样的解释，即无论是用PHA还是用淋巴因子激活的免疫活性淋巴细胞都可能刺激脾集落生长和红系分化，尽管程度不同。
