Sekiya N, Tanaka N, Itoh T, Shimada Y, Goto H, Terasawa K
Department of Japanese Oriental Medicine, Toyama Medical and Pharmaceutical University, Japan.
Phytother Res. 1999 May;13(3):192-6. doi: 10.1002/(SICI)1099-1573(199905)13:3<192::AID-PTR412>3.0.CO;2-2.
In this study, we examined whether in vivo keishi-bukuryo-gan (a Kampo formulation) could prevent the progression of atherosclerosis in cholesterol-fed rabbits, an animal model for hypercholesterolaemia. Sixteen male Japanese white rabbits (2 kg body weight) were divided into two groups. Group A (n = 8) was fed standard rabbit chow containing 1% cholesterol for 8 weeks. Group B (n = 8) was fed standard rabbit chow containing 1% cholesterol and 1% keishi-bukuryo-gan for 8 weeks. At the end of the experiment, average plasma concentrations of total-cholesterol and IDL-cholesterol were 2055.9 +/- 201.8 mg/dL and 408.1 +/- 62.6 mg/dL in group A and 1950.5 +/- 126.3 mg/dL and 407.6 +/- 56.6 mg/dL in group B, respectively. The percentage of the surface area of the total thoracic aorta with visible plaque was significantly reduced by keishi-bukuryo-gan administration; group A was 33.2% +/- 5.3% and group B was 14.3% +/- 2.9%. beta-very low density lipoprotein (VLDL) and low density lipoprotein (LDL) isolated from cholesterol fed rabbits treated with keishi-bukuryo-gan (group B) were shown to be highly resistant to oxidative modification by cupric ion. Sera isolated from rabbits administered keishi-bukuryo-gan had reduced lipid peroxide formation compared with those from rabbits without keishi-bukuryo-gan. Thus, keishi-bukuryo-gan prevents the progression of atherosclerosis in cholesterol-fed rabbits in vivo by limiting oxidative LDL modification.
在本研究中，我们检测了体内给予桂枝茯苓丸（一种汉方制剂）是否能预防高胆固醇血症动物模型——胆固醇喂养兔的动脉粥样硬化进展。将16只雄性日本白兔（体重2千克）分为两组。A组（n = 8）给予含1%胆固醇的标准兔饲料，持续8周。B组（n = 8）给予含1%胆固醇和1%桂枝茯苓丸的标准兔饲料，持续8周。实验结束时，A组总胆固醇和中间密度脂蛋白胆固醇的平均血浆浓度分别为2055.9±201.8毫克/分升和408.1±62.6毫克/分升，B组分别为1950.5±126.3毫克/分升和407.6±56.6毫克/分升。给予桂枝茯苓丸显著降低了可见斑块的胸主动脉总表面积百分比；A组为33.2%±5.3%，B组为14.3%±2.9%。从给予桂枝茯苓丸的胆固醇喂养兔（B组）分离出的β-极低密度脂蛋白（VLDL）和低密度脂蛋白（LDL）对铜离子介导的氧化修饰具有高度抗性。与未给予桂枝茯苓丸的兔相比，给予桂枝茯苓丸的兔血清中脂质过氧化物形成减少。因此，桂枝茯苓丸通过限制氧化型低密度脂蛋白的修饰，在体内预防胆固醇喂养兔的动脉粥样硬化进展。
