通过随机扩增多态性DNA分析比较人类和犰狳巴西副球孢子菌分离株
Comparison between human and armadillo Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis.
作者信息
Sano A, Tanaka R, Yokoyama K, Franco M, Bagagli E, Montenegro M R, Mikami Y, Miyaji M, Nishimura K
机构信息
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.
出版信息
Mycopathologia. 1998;143(3):165-9. doi: 10.1023/a:1006949113529.
Sixty-three Paracoccidioides brasiliensis isolates obtained from three nine-banded armadillos (Dasypus novemcinctus), one Amazonian armadillo's and 19 clinical isolates were compared by random amplified polymorphic DNA analysis with the primer OPG-19. The isolates were divided into three major clusters, I, II and III. Coincidences between human and armadillo isolates were observed in clusters I and II. Cluster III consisted only of armadillos' isolates. The results suggested that (I) humans may acquire P. brasiliensis infection by contact with armadillo's environment, (II) there may be P. brasiliensis genotypes peculiar to the animal, and (III) individual armadillos may be infected with P. brasiliensis cells with different genotypes.
采用引物OPG - 19，通过随机扩增多态性DNA分析，比较了从三只九带犰狳（Dasypus novemcinctus）、一只亚马逊犰狳获得的63株巴西副球孢子菌分离株以及19株临床分离株。这些分离株被分为三个主要类群，即I、II和III。在类群I和II中观察到人类和犰狳分离株之间存在一致性。类群III仅由犰狳的分离株组成。结果表明：（I）人类可能通过接触犰狳的环境而感染巴西副球孢子菌；（II）可能存在该动物特有的巴西副球孢子菌基因型；（III）个体犰狳可能感染了不同基因型的巴西副球孢子菌细胞。
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