通过随机扩增多态性DNA分析对巴西副球孢子菌分离株进行鉴定。
Characterization of Paracoccidioides brasiliensis isolates by random amplified polymorphic DNA analysis.
作者信息
Soares C M, Madlun E E, da Silva S P, Pereira M, Felipe M S
机构信息
Laboratório de Biologia Molecular, IB, Universidade de Brasília, Distrito Federal.
出版信息
J Clin Microbiol. 1995 Feb;33(2):505-7. doi: 10.1128/jcm.33.2.505-507.1995.
Abstract
We initially used 25 different random primers in order to test their ability to generate random amplified polymorphic DNA fragments from the dimorphic human pathogenic fungus Paracoccidioides brasiliensis. From the tested primers we chose five to distinguish between seven isolates of this microorganism. The DNA amplification patterns allowed clear differentiation of the seven isolates into two distinct groups with only 35% genomic identity. One of these groups contained two subgroups with 81% genetic similarity. The random amplified polymorphic DNA analysis method proved to be a good tool for analyzing and comparing different genomes of P. brasiliensis isolates.
摘要
我们最初使用了25种不同的随机引物，以测试它们从双态性人类致病真菌巴西副球孢子菌中产生随机扩增多态性DNA片段的能力。从测试的引物中，我们选择了5种来区分该微生物的7个分离株。DNA扩增模式能够将这7个分离株清晰地分为两个不同的组，它们只有35%的基因组同一性。其中一组包含两个亚组，遗传相似性为81%。随机扩增多态性DNA分析方法被证明是分析和比较巴西副球孢子菌分离株不同基因组的良好工具。
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