Kayastha S, Freedman A N, Piver M S, Mukkamalla J, Romero-Guittierez M, Werness B A
Division of Pathology, Roswell Park Cancer Institute, Buffalo, New York 14263, USA.
Clin Cancer Res. 1999 May;5(5):1073-6.
Most ovarian carcinomas present at advanced stage, principally as the result of dissemination to peritoneal sites. Standard CD44 (CD44S) is the principal receptor for hyaluronic acid, and in vitro and animal studies have suggested that the attachment of ovarian carcinoma cells to the peritoneal mesothelium involves the interaction between CD44S on ovarian carcinoma cells and hyaluronic acid on mesothelial surfaces. We, therefore, analyzed a series of ovarian carcinomas for the expression of CD44S by immunohistochemistry to see whether expression of this receptor by tumor cells correlated with clinicopathological factors and measures of patient outcome. Fifty-six fixed, paraffin-embedded primary epithelial ovarian tumors were immunostained with antibody to CD44S. Membrane staining was considered positive, and results were correlated with stage, grade, age, histology, and survival. Twenty-two (39%) tumors were positive for CD44S. There was no correlation between CD44 expression and histological type, grade, age, or stage. However, CD44 expression was significantly associated with survival in both univariate (P = 0.003) and multivariate (P = 0.006) analyses. These results support a role for CD44S expression in the spread of ovarian epithelial cancer and suggest that expression of this molecule is a significant independent predictor of survival in women with this disease.
大多数卵巢癌在晚期出现，主要是由于癌细胞扩散至腹膜部位所致。标准CD44（CD44S）是透明质酸的主要受体，体外和动物研究表明，卵巢癌细胞与腹膜间皮的黏附涉及卵巢癌细胞上的CD44S与间皮表面透明质酸之间的相互作用。因此，我们通过免疫组织化学分析了一系列卵巢癌中CD44S的表达情况，以观察肿瘤细胞中该受体的表达是否与临床病理因素及患者预后指标相关。用抗CD44S抗体对56例固定的石蜡包埋原发性上皮性卵巢肿瘤进行免疫染色。细胞膜染色被视为阳性，并将结果与分期、分级、年龄、组织学类型及生存率进行关联分析。22例（39%）肿瘤CD44S呈阳性。CD44表达与组织学类型、分级、年龄或分期之间无相关性。然而，在单因素分析（P = 0.003）和多因素分析（P = 0.006）中，CD44表达均与生存率显著相关。这些结果支持CD44S表达在卵巢上皮癌扩散中发挥作用，并表明该分子的表达是患有这种疾病女性生存率的一个重要独立预测指标。
