Isoherranen K, Punnonen K, Jansen C, Uotila P
Department of Clinical Chemistry, University of Turku, Turku, Finland.
Br J Dermatol. 1999 Jun;140(6):1017-22. doi: 10.1046/j.1365-2133.1999.02897.x.
We examined the effect of ultraviolet (UV) irradiation on the expression of cyclooxygenases in cultured HaCaT keratinocytes and in human skin in vivo. UVB irradiation (10 and 50 mJ/cm2) and hydrogen peroxide (200 micromol/L) increased cyclooxygenase-2 mRNA expression in HaCaT keratinocytes. No clear expression of cyclooxygenase-1 mRNA was detected in either control or stimulated HaCaT cells. Genistein, a tyrosine kinase inhibitor, suppressed both the basal and stimulated expression of cyclooxygenase-2 in HaCaT cells. UVB-induced cyclooxygenase-2 mRNA expression was partly inhibited by the antioxidant N-acetylcysteine and by H-7, a non-specific inhibitor of protein kinase C. Solar-simulated irradiation (40 mJ/cm2) was found to induce in vivo both cyclooxygenase-2 mRNA and protein expression in human skin, whereas the expression of cyclooxygenase-1 mRNA remained at the basal level. Our results show that cyclooxygenase-2 expression is induced by UV irradiation and suggest that tyrosine kinases and reactive oxygen intermediates are involved in this induction of cyclooxygenase-2.
我们研究了紫外线（UV）照射对体外培养的HaCaT角质形成细胞以及人体皮肤中环氧化酶表达的影响。紫外线B（UVB）照射（10和50 mJ/cm²）以及过氧化氢（200 μmol/L）均可增加HaCaT角质形成细胞中环氧化酶-2的mRNA表达。在对照或受刺激的HaCaT细胞中均未检测到环氧化酶-1 mRNA的明显表达。酪氨酸激酶抑制剂染料木黄酮可抑制HaCaT细胞中环氧化酶-2的基础表达和受刺激后的表达。抗氧化剂N-乙酰半胱氨酸以及蛋白激酶C的非特异性抑制剂H-7可部分抑制UVB诱导的环氧化酶-2 mRNA表达。研究发现，模拟阳光照射（40 mJ/cm²）可在人体皮肤中诱导环氧化酶-2的mRNA和蛋白表达，而环氧化酶-1 mRNA的表达则维持在基础水平。我们的结果表明，UV照射可诱导环氧化酶-2表达，并提示酪氨酸激酶和活性氧中间体参与了环氧化酶-2的这一诱导过程。
