Seifert J, Hallfeld K, Eberle B
Chirurgische Forschung der Klinik für Allgemeine, Christian-Albrechts-Universität Kiel.
Zentralbl Chir. 1999;124(4):292-7.
Numerous antigens are offered to the living organism by the daily food. With these investigations the influence of orally administered human gammaglobulin (HGG) on the immunity of sensitized animals after systemic immunization should be clarified. Experiments were performed in rabbits. Changes of the circulation were measured by bloodpressure registration and measurements of the organ flow. Simultaneous investigations of the antibody content, complement C3 and histamin were performed in the peripheral blood. In orally fed animals as well as in non fed controls an i.v. antigen challenge was performed. The level of circulating antibodies could be significantly decreased (60%) by the oral administration of HGG. In control animals the parenteral antigen challenge resulted in a mortality rate of 50%. In these animals the bloodpressure decreased significantly and long lasting, whereas antigen fed animals showed only a slight change in bloodpressure with normalization after a short period. During oral absorption of antigen complement factor C3 remained unchanged. In the first hour after the oral antigen administration a significant increase of histamin in the peripheral blood could be observed. Investigation with regard to the fate of the antibodies which were decreased by antigen feeding showed a storage in the layers of the gut which was depending on the dosage. It could be demonstrated that it is possible to influence the systemic immune response by the enteral application of macromolecules and this is also relevant for reactions of the circulation.
日常食物为生物体提供了众多抗原。通过这些研究，应阐明口服人丙种球蛋白（HGG）对致敏动物全身免疫后的免疫力的影响。实验在兔子身上进行。通过血压记录和器官血流测量来测定循环变化。同时对外周血中的抗体含量、补体C3和组胺进行研究。对口服给药的动物以及未给药的对照动物进行静脉内抗原激发。口服HGG可使循环抗体水平显著降低（60%）。在对照动物中，胃肠外抗原激发导致死亡率为50%。在这些动物中，血压显著且持久下降，而喂食抗原的动物血压仅出现轻微变化，并在短时间后恢复正常。在口服抗原吸收过程中，补体因子C3保持不变。在口服抗原给药后的第一小时，可观察到外周血中组胺显著增加。关于因喂食抗原而减少的抗体的去向研究表明，其在肠道层中的储存量取决于剂量。可以证明，通过肠道应用大分子能够影响全身免疫反应，这对于循环反应也具有相关性。
