Odagiri S
Department of Rheumatology, Saitama Medical School.
Arerugi. 1999 Apr;48(4):451-8.
The function of dendritic cell (DC), that has the strongest antigen-presenting ability, was investigated for the purpose of clarifying immune abnormalities in Sjögren's syndrome (SS) from the point of view of antigen-presenting cells. DCs were separated from peripheral blood of SS patients, and mixed lymphocyte reaction (MLR) with DCs was observed to examine the function of DC. Autologous MLR (AMLR), which was induced by mixed culture of DCs with autologous T cells, significantly decreased in SS patients (p < 0.01). Allogeneic MLR (alloMLR), which was induced by mixed culture of DCs with allogeneic T cells, also decreased in SS patients, suggesting the presence of DC dysfunction. Cell surface expression rates of HLA-DR, CD 80, and CD 83, which are a first signal of cell surface antigen presentation, a second signal, and a specific antigen of DC, respectively, were then examined. Among HLA-DR-positive cells, CD 80-positive cells and CD 83-positive cells increased in SS patients, compared with normal subjects (p < 0.0003 and p < 0.00009, respectively). From these results, it was inferred that SS patients have not only DC dysfunction, but abnormalities in cell surface antigens as well.
为了从抗原呈递细胞的角度阐明干燥综合征（SS）中的免疫异常，研究了具有最强抗原呈递能力的树突状细胞（DC）的功能。从SS患者的外周血中分离出DC，并观察DC的混合淋巴细胞反应（MLR）以检查DC的功能。由DC与自体T细胞混合培养诱导的自体MLR（AMLR）在SS患者中显著降低（p < 0.01）。由DC与异体T细胞混合培养诱导的异体MLR（alloMLR）在SS患者中也降低，提示存在DC功能障碍。然后检查了HLA-DR、CD 80和CD 83的细胞表面表达率，它们分别是细胞表面抗原呈递的第一信号、第二信号和DC的特异性抗原。在HLA-DR阳性细胞中，与正常受试者相比，SS患者中CD 80阳性细胞和CD 83阳性细胞增加（分别为p < 0.0003和p < 0.00009）。从这些结果推断，SS患者不仅存在DC功能障碍，而且细胞表面抗原也存在异常。
