Prester L, Juresa D, Blanusa M
Institute for Medical Research and Occupational Health, Zagreb, Croatia.
Arh Hig Rada Toksikol. 1998 Dec;49(4):343-8.
The aim of the study was to compare the efficiency of acid and alkaline decomposition of biological materials using an open and a closed system for total mercury determination. Acid digestion was performed with concentrated HNO3 in tubes at 80 degrees C and lasted five hours. Alkaline digestion was performed with a 45% NaOH and a 1% cysteine, heated at 120 degrees C for 20 minutes. Total mercury was measured by atomic absorption spectrometry using the cold vapour technique (CVAAS). The average recovery obtained for analysis of certified reference material in closed tubes for acid digested sample was superior to the alkaline one, 103 +/- 4% vs. 70 +/- 3%, respectively. In addition, the recoveries through the open system acid digestion (90 +/- 8%) and the open system alkaline digestion (57 +/- 2%) were lower than through the respective closed system digestions. Reproducibility of the acid decomposition method was superior to the alkaline one.
本研究的目的是比较使用开放和封闭系统进行生物材料中总汞测定时酸分解和碱分解的效率。酸消解在试管中于80℃用浓硝酸进行，持续5小时。碱消解用45%的氢氧化钠和1%的半胱氨酸进行，在120℃加热20分钟。总汞采用冷蒸气技术（CVAAS）通过原子吸收光谱法测定。酸消解样品在封闭试管中分析认证参考物质时获得的平均回收率优于碱消解，分别为103±4%和70±3%。此外，开放系统酸消解（90±8%）和开放系统碱消解（57±2%）的回收率低于各自的封闭系统消解。酸分解方法的重现性优于碱分解方法。
