一种用于测量超突变率时解决卢里亚-德尔布吕克波动问题的实验性解决方案。
An experimental solution for the Luria-Delbrück fluctuation problem in measuring hypermutation rates.
作者信息
Bachl J, Dessing M, Olsson C, von Borstel R C, Steinberg C
机构信息
Basel Institute for Immunology, Postfach, CH-4005 Basel, Switzerland.
出版信息
Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):6847-9. doi: 10.1073/pnas.96.12.6847.
Abstract
A cell line harboring all trans-acting elements necessary for hypermutation was transfected with a plasmid harboring the major cis-acting elements plus a green fluorescent protein gene containing a premature chain-termination codon. Transfected cells do not fluoresce unless the stop codon reverts. When a sizable cell population is purged of revertants by sorting, the frequency of mutants increases linearly with time, and there is no Luria-Delbrück fluctuation effect. Moreover, as mutant frequencies seemed to vary less than cell numbers in replicate cultures, it is suggested that hypermutation might not be coupled closely to cell division.
摘要
将携带高突变所需所有反式作用元件的细胞系用携带主要顺式作用元件的质粒以及含有提前链终止密码子的绿色荧光蛋白基因进行转染。除非终止密码子回复突变，否则转染的细胞不会发出荧光。当通过分选清除相当数量的回复突变体后，突变体的频率随时间呈线性增加，并且不存在卢里亚-德尔布吕克波动效应。此外，由于在重复培养中突变频率的变化似乎小于细胞数量的变化，因此有人提出高突变可能与细胞分裂没有紧密联系。
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本文引用的文献
1
The Direct Estimation of Mutation Rate from Mutant Frequency under Special Conditions.在特殊条件下根据突变体频率直接估计突变率
Genetics. 1959 May;44(3):483-96. doi: 10.1093/genetics/44.3.483.
2
Mutations of Bacteria from Virus Sensitivity to Virus Resistance.细菌从对病毒敏感到对病毒抗性的突变。
Genetics. 1943 Nov;28(6):491-511. doi: 10.1093/genetics/28.6.491.
3
Experiments with the Chemostat on spontaneous mutations of bacteria.使用恒化器对细菌自发突变进行的实验。
Proc Natl Acad Sci U S A. 1950 Dec;36(12):708-19. doi: 10.1073/pnas.36.12.708.
4
Direct demonstration of the mutagenic action of euflavine on baker's yeast.直接证明优黄素对面包酵母的诱变作用。
Nature. 1950 Dec 2;166(4231):956. doi: 10.1038/166956a0.
5
Description of the chemostat.恒化器的描述。
Science. 1950 Dec 15;112(2920):715-6. doi: 10.1126/science.112.2920.715.
6
Probing immunoglobulin gene hypermutation with microsatellites suggests a nonreplicative short patch DNA synthesis process.利用微卫星探究免疫球蛋白基因超突变表明存在一种非复制性短片段DNA合成过程。
Immunity. 1998 Aug;9(2):257-65. doi: 10.1016/s1074-7613(00)80608-1.
7
A novel bacterial reversion and forward mutation assay based on green fluorescent protein.一种基于绿色荧光蛋白的新型细菌回复突变和正向突变检测方法。
Mutat Res. 1998 May 11;414(1-3):95-105. doi: 10.1016/s1383-5718(98)00043-6.
8
The Ig mutator is dependent on the presence, position, and orientation of the large intron enhancer.免疫球蛋白突变体依赖于大型内含子增强子的存在、位置和方向。
Proc Natl Acad Sci U S A. 1998 Mar 3;95(5):2396-9. doi: 10.1073/pnas.95.5.2396.
9
Mismatch repair as a source of mutations in non-dividing cells.错配修复作为非分裂细胞中突变的一个来源。
Genetica. 1996 Mar;97(2):183-95. doi: 10.1007/BF00054625.
10
Enhancers of hypermutation.高突变增强子。
Immunogenetics. 1996;45(1):59-64. doi: 10.1007/s002510050167.
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