Gupta K, Bewtra A
Creighton University, Department of Medicine/Division of Allergy, Omaha, Nebraska, USA.
J Allergy Clin Immunol. 1999 Jun;103(6):1100-7. doi: 10.1016/s0091-6749(99)70185-5.
IL-1 has 2 receptors, type I (IL-1RI) and type II (IL-1RII), which have 2 forms each, membrane (m) and soluble (s). When IL-1 binds to mIL-1RI, the active receptor, an inflammatory response is initiated, which does not occur when IL-1 binds to mIL-1RII, the decoy receptor. Both sIL-1RI and sIL-1RII function as IL-1-mopping mechanisms. We hypothesized that the ratio of active (mIL-1RI) to inactive (mIL-1RII, sIL-1RI, and sIL-1RII) receptors is important in determining the amount of inflammation produced in allergic reactions.
Our aim was to compare the concentrations of mIL-1RI and mIL-1RII on cultured PBLs and sIL-1RI, sIL-1RII, and IL-1beta in sera and supernatants of cultured PBMCs from atopic and nonatopic subjects.
The membrane receptors, soluble receptors, and IL-1beta concentrations were measured by ELISA with specific mAbs.
Although there was no difference in the level of serum IL-1beta between the 2 groups, PBMCs from atopic persons spontaneously secreted higher levels of IL-1beta than those from nonatopic donors (P < .05). PBLs from atopic subjects compared with those from nonatopic individuals expressed higher mIL-1RI (P < .0001) and mIL-1RII (P < .05). Levels of both the soluble receptors from both serum (P < .0001) and PBMCs (P < .05) of nonatopic donors were higher than those found in atopic donors.
This augmentation of mIL-1RI concomitant with a reduction in soluble receptors may be an important contributory factor to the inflammation that occurs with allergen exposure.
白细胞介素-1（IL-1）有两种受体，即I型（IL-1RI）和II型（IL-1RII），它们各自有两种形式，膜结合型（m）和可溶性（s）。当IL-1与活性受体mIL-1RI结合时，会引发炎症反应，而当IL-1与诱饵受体mIL-1RII结合时则不会发生这种情况。sIL-1RI和sIL-1RII都起到清除IL-1的作用。我们推测活性受体（mIL-1RI）与非活性受体（mIL-1RII、sIL-1RI和sIL-1RII）的比例对于确定过敏反应中产生的炎症量很重要。
我们的目的是比较特应性和非特应性受试者培养的外周血淋巴细胞（PBLs）上mIL-1RI和mIL-1RII的浓度，以及培养的外周血单核细胞（PBMCs）的血清和上清液中sIL-1RI、sIL-1RII和IL-1β的浓度。
用特异性单克隆抗体通过酶联免疫吸附测定法（ELISA）测量膜受体、可溶性受体和IL-1β的浓度。
虽然两组之间血清IL-1β水平没有差异，但特应性个体的PBMCs自发分泌的IL-1β水平高于非特应性供体（P <.05）。与非特应性个体相比，特应性受试者的PBLs表达更高的mIL-1RI（P <.0001）和mIL-1RII（P <.05）。非特应性供体血清（P <.0001）和PBMCs（P <.05）中可溶性受体的水平均高于特应性供体。
mIL-1RI的这种增加伴随着可溶性受体的减少可能是过敏原暴露时发生炎症的一个重要促成因素。
