Yu P W, Schuler L A, Czuprynski C J
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 53706, USA.
Clin Diagn Lab Immunol. 1997 Nov;4(6):769-73. doi: 10.1128/cdli.4.6.769-773.1997.
Interleukin-1 (IL-1) is a key player in inflammation and the immune response. To better understand the complex interactions of IL-1 and its receptors in inflammation, we need to investigate how type I and type II IL-1 receptors (IL-1RI and IL-1RII) are regulated by cytokines and other mediators. Using semiquantitative reverse transcriptase PCR and Northern analysis, we examined the regulation of IL-1RI and IL-1RII mRNA levels in bovine polymorphonuclear leukocytes (PMNs) (i.e., neutrophils) and peripheral blood mononuclear cells (PBMCs) in vitro. IL-1RI mRNA levels were up-regulated in PBMCs by recombinant bovine IL-1beta (rBoIL-1beta), recombinant bovine granulocyte-macrophage colony-stimulating factor (rBoGM-CSF), rBoIL-4, recombinant bovine gamma interferon (rBoIFN-gamma), and dexamethasone. IL-1RI mRNA was increased in bovine PMNs exposed to rBoGM-CSF, rBoIL-4, and dexamethasone but was down-regulated by rBoIL-1beta and rBoIFN-gamma. IL-1RII mRNA was increased in bovine PBMCs and PMNs after exposure to rBoIL-1beta, rBoGM-CSF, rBoIL-4, and dexamethasone. In contrast, rBoIFN-gamma down-regulated the expression of bovine IL-1RII mRNA in PBMCs. These findings suggest that the expression of bovine IL-1RI and IL-1RII mRNAs is regulated differently by certain soluble stimuli (e.g., IFN-gamma) in PMNs and PBMCs.
白细胞介素-1（IL-1）是炎症和免疫反应中的关键因子。为了更好地理解IL-1及其受体在炎症中的复杂相互作用，我们需要研究I型和II型白细胞介素-1受体（IL-1RI和IL-1RII）是如何受到细胞因子和其他介质调节的。我们使用半定量逆转录酶PCR和Northern分析，在体外检测了牛多形核白细胞（PMNs，即中性粒细胞）和外周血单核细胞（PBMCs）中IL-1RI和IL-1RII mRNA水平的调节情况。重组牛IL-1β（rBoIL-1β）、重组牛粒细胞-巨噬细胞集落刺激因子（rBoGM-CSF）、rBoIL-4、重组牛γ干扰素（rBoIFN-γ）和地塞米松可使PBMCs中的IL-1RI mRNA水平上调。暴露于rBoGM-CSF、rBoIL-4和地塞米松的牛PMNs中IL-1RI mRNA增加，但rBoIL-1β和rBoIFN-γ可使其下调。暴露于rBoIL-1β、rBoGM-CSF、rBoIL-4和地塞米松后，牛PBMCs和PMNs中的IL-1RII mRNA增加。相反，rBoIFN-γ可下调PBMCs中牛IL-1RII mRNA的表达。这些发现表明牛IL-1RI和IL-1RII mRNA的表达在PMNs和PBMCs中受到某些可溶性刺激（如IFN-γ）的不同调节。
