通过聚合酶链反应快速鉴定汉逊德巴利酵母/法塔假丝酵母
Rapid identification of Debaryomyces hansenii/Candida famata by polymerase chain reaction.
作者信息
Nishikawa A, Sugita T, Shinoda T
机构信息
Department of Immunobiology, Meiji Pharmaceutical University, Tokyo, Japan.
出版信息
Med Mycol. 1999 Apr;37(2):101-4.
PMID:10361265
Abstract
Primers designed in this study were used in a polymerase chain reaction test to amplify a species-specific fragment of approximately 340 bp of the large subunit ribosomal DNA of Debaryomyces hansenii/Candida famata. None of the other medically relevant yeasts including C. guilliermondii, and also the related species, D. nepalensis and C. saitoana, were amplified by this primer pair.
摘要
本研究设计的引物用于聚合酶链反应试验，以扩增汉逊德巴利酵母/法塔假丝酵母大亚基核糖体DNA约340 bp的物种特异性片段。包括季也蒙假丝酵母在内的其他医学相关酵母，以及相关物种尼泊尔德巴利酵母和斋藤假丝酵母，均未被该引物对扩增。
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