重组腺相关病毒对分化良好的气道上皮的转导受载体进入的限制。
Transduction of well-differentiated airway epithelium by recombinant adeno-associated virus is limited by vector entry.
作者信息
Bals R, Xiao W, Sang N, Weiner D J, Meegalla R L, Wilson J M
机构信息
Departments of Medicine and Molecular and Cellular Engineering, Institute for Human Gene Therapy, and The Wistar Institute, Philadelphia, Pennsylvania 19104, USA.
出版信息
J Virol. 1999 Jul;73(7):6085-8. doi: 10.1128/JVI.73.7.6085-6088.1999.
Abstract
The limitations of adeno-associated virus (AAV)-mediated vectors for lung-directed gene transfer were investigated by using differentiated human respiratory epithelium in air-liquid interface cultures. Transduction efficiency was high in undifferentiated cells and was enhanced in well-differentiated cells after basolateral application of the vector or after apical application following disruption of tight junctions or pretreatment of the cultures with glycosidases. These results indicate that transduction of airway epithelia by AAV vectors is limited by entry and reinforce the importance of a physical barrier on the airway surface.
摘要
通过在气液界面培养中使用分化的人呼吸道上皮细胞，研究了腺相关病毒（AAV）介导的载体用于肺定向基因转移的局限性。在未分化细胞中，转导效率很高；在载体从基底外侧应用后，或在紧密连接破坏后从顶端应用载体，或用糖苷酶预处理培养物后，在分化良好的细胞中转导效率会提高。这些结果表明，AAV载体对气道上皮细胞的转导受进入限制，并强化了气道表面物理屏障的重要性。
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本文引用的文献
1
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Nat Med. 1999 Jan;5(1):71-7. doi: 10.1038/4758.
2
Adeno-associated virus as a vector for liver-directed gene therapy.腺相关病毒作为肝脏定向基因治疗的载体。
J Virol. 1998 Dec;72(12):10222-6. doi: 10.1128/JVI.72.12.10222-10226.1998.
3
Influence of cell polarity on retrovirus-mediated gene transfer to differentiated human airway epithelia.细胞极性对逆转录病毒介导的基因转移至分化的人气道上皮细胞的影响。
J Virol. 1998 Dec;72(12):9818-26. doi: 10.1128/JVI.72.12.9818-9826.1998.
4
Factors influencing adeno-associated virus-mediated gene transfer to human cystic fibrosis airway epithelial cells: comparison with adenovirus vectors.影响腺相关病毒介导的基因转移至人囊性纤维化气道上皮细胞的因素：与腺病毒载体的比较。
J Virol. 1998 Nov;72(11):8904-12. doi: 10.1128/JVI.72.11.8904-8912.1998.
5
Efficient and persistent gene transfer of AAV-CFTR in maxillary sinus.腺相关病毒介导的囊性纤维化跨膜传导调节因子在鼻窦中的高效持久基因转移
Lancet. 1998 Jun 6;351(9117):1702-3. doi: 10.1016/S0140-6736(05)77740-0.
6
Adeno-associated virus vectors for gene therapy of cystic fibrosis.用于囊性纤维化基因治疗的腺相关病毒载体。
Methods Enzymol. 1998;292:717-32. doi: 10.1016/s0076-6879(98)92055-9.
7
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J Virol. 1998 Jul;72(7):6014-23. doi: 10.1128/JVI.72.7.6014-6023.1998.
8
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J Virol. 1998 May;72(5):4212-23. doi: 10.1128/JVI.72.5.4212-4223.1998.
9
Lentiviral vectors for gene therapy of cystic fibrosis.用于囊性纤维化基因治疗的慢病毒载体。
Hum Gene Ther. 1997 Dec 10;8(18):2261-8. doi: 10.1089/hum.1997.8.18-2261.
10
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J Virol. 1998 Feb;72(2):1438-45. doi: 10.1128/JVI.72.2.1438-1445.1998.
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