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真核生物起始因子eIF4B与口蹄疫病毒内部核糖体进入位点的相互作用不依赖于多嘧啶序列结合蛋白。

Interaction of eukaryotic initiation factor eIF4B with the internal ribosome entry site of foot-and-mouth disease virus is independent of the polypyrimidine tract-binding protein.

作者信息

Rust R C, Ochs K, Meyer K, Beck E, Niepmann M

机构信息

Institute of Biochemistry, D-35392 Giessen, Germany.

出版信息

J Virol. 1999 Jul;73(7):6111-3. doi: 10.1128/JVI.73.7.6111-6113.1999.

DOI:10.1128/JVI.73.7.6111-6113.1999
PMID:10364367
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC112676/
Abstract

Eukaryotic translation initiation factor 4B (eIF4B) binds directly to the internal ribosome entry site (IRES) of foot-and-mouth disease virus (FMDV). Mutations in all three subdomains of the IRES stem-loop 4 reduce binding of eIF4B and translation efficiency in parallel, indicating that eIF4B is functionally involved in FMDV translation initiation. In reticulocyte lysate devoid of polypyrimidine tract-binding protein (PTB), eIF4B still bound well to the wild-type IRES, even after removal of the major PTB-binding site. In conclusion, the interaction of eIF4B with the FMDV IRES is essential for IRES function but independent of PTB.

摘要

真核生物翻译起始因子4B（eIF4B）直接与口蹄疫病毒（FMDV）的内部核糖体进入位点（IRES）结合。IRES茎环4的所有三个亚结构域中的突变会同时降低eIF4B的结合以及翻译效率，这表明eIF4B在功能上参与了FMDV的翻译起始过程。在缺乏聚嘧啶序列结合蛋白（PTB）的网织红细胞裂解物中，即使去除了主要的PTB结合位点，eIF4B仍能很好地与野生型IRES结合。总之，eIF4B与FMDV IRES的相互作用对于IRES功能至关重要，但独立于PTB。

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本文引用的文献

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Functional involvement of polypyrimidine tract-binding protein in translation initiation complexes with the internal ribosome entry site of foot-and-mouth disease virus.多嘧啶序列结合蛋白在与口蹄疫病毒内部核糖体进入位点形成的翻译起始复合物中的功能作用。
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Structural analysis of the interaction of the pyrimidine tract-binding protein with the internal ribosomal entry site of encephalomyocarditis virus and foot-and-mouth disease virus RNAs.嘧啶序列结合蛋白与脑心肌炎病毒及口蹄疫病毒RNA的内部核糖体进入位点相互作用的结构分析
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Canonical eukaryotic initiation factors determine initiation of translation by internal ribosomal entry.经典的真核生物起始因子通过核糖体内部进入来决定翻译起始。
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Direct evidence that polypyrimidine tract binding protein (PTB) is essential for internal initiation of translation of encephalomyocarditis virus RNA.多嘧啶序列结合蛋白（PTB）对脑心肌炎病毒RNA翻译的内部起始至关重要的直接证据。
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