真核生物起始因子eIF4B与口蹄疫病毒内部核糖体进入位点的相互作用不依赖于多嘧啶序列结合蛋白。
Interaction of eukaryotic initiation factor eIF4B with the internal ribosome entry site of foot-and-mouth disease virus is independent of the polypyrimidine tract-binding protein.
作者信息
Rust R C, Ochs K, Meyer K, Beck E, Niepmann M
机构信息
Institute of Biochemistry, D-35392 Giessen, Germany.
出版信息
J Virol. 1999 Jul;73(7):6111-3. doi: 10.1128/JVI.73.7.6111-6113.1999.
Abstract
Eukaryotic translation initiation factor 4B (eIF4B) binds directly to the internal ribosome entry site (IRES) of foot-and-mouth disease virus (FMDV). Mutations in all three subdomains of the IRES stem-loop 4 reduce binding of eIF4B and translation efficiency in parallel, indicating that eIF4B is functionally involved in FMDV translation initiation. In reticulocyte lysate devoid of polypyrimidine tract-binding protein (PTB), eIF4B still bound well to the wild-type IRES, even after removal of the major PTB-binding site. In conclusion, the interaction of eIF4B with the FMDV IRES is essential for IRES function but independent of PTB.
摘要
真核生物翻译起始因子4B（eIF4B）直接与口蹄疫病毒（FMDV）的内部核糖体进入位点（IRES）结合。IRES茎环4的所有三个亚结构域中的突变会同时降低eIF4B的结合以及翻译效率，这表明eIF4B在功能上参与了FMDV的翻译起始过程。在缺乏聚嘧啶序列结合蛋白（PTB）的网织红细胞裂解物中，即使去除了主要的PTB结合位点，eIF4B仍能很好地与野生型IRES结合。总之，eIF4B与FMDV IRES的相互作用对于IRES功能至关重要，但独立于PTB。
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本文引用的文献
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