通过在可控孔径玻璃上进行吸附色谱法纯化干扰素。
Purification of interferon by adsorption chromatography on controlled pore glass.
作者信息
Edy V G, Braude I A, De Clercq E, Billiau A, De Somer P
出版信息
J Gen Virol. 1976 Dec;33(3):517-21. doi: 10.1099/0022-1317-33-3-517.
PMID:1036458
Abstract
Human fibroblast and mouse L929 cell interferons can be purified by adsoprtion to and subsequent elution from Controlled Pore Glass. Purification of 40 to 90-fold to specific activities of 1 to 5 times 10(6) units/mg of protein can be achieved in a single step, with good recovery of activity. Human leukocyte interferon does not bind to the glass and cannot be purified in this way.
摘要
人成纤维细胞干扰素和小鼠L929细胞干扰素可通过吸附到可控孔径玻璃上并随后洗脱来纯化。在一步操作中可实现40至90倍的纯化，使比活性达到1至5×10⁶单位/毫克蛋白质，且活性回收率良好。人白细胞干扰素不与玻璃结合，无法用这种方法纯化。
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