Van Looveren M, Ison C A, Ieven M, Vandamme P, Martin I M, Vermeulen K, Renton A, Goossens H
Department of Medical Microbiology, University Hospital Antwerp, University of Antwerp, Antwerp, Belgium.
J Clin Microbiol. 1999 Jul;37(7):2183-8. doi: 10.1128/JCM.37.7.2183-2188.1999.
A panel of 18 strains of Neisseria gonorrhoeae, known to be temporally and geographically diverse, was used to evaluate a number of typing systems, including conventional auxotyping and serotyping and the molecular methods of arbitrarily primed PCR (AP-PCR), amplified ribosomal-DNA restriction analysis (ARDRA), opa typing, and pulsed-field gel electrophoresis (PFGE). The discriminatory power of the different typing methods were determined with a collection of 87 clinical isolates from commercial sex workers in Indonesia, and Simpson's index of diversity was calculated. Of the two traditional techniques, auxotyping and serotyping, the latter gives the highest discriminatory index (DI) (DI, 0.846). The combination of auxotyping and serotyping yields a high DI (DI, 0. 928). D11344- and D8635-primed PCR showed low DIs of 0.608 and 0.622, respectively, but a combination of the two primers had a DI of 0. 849. The combination of serotyping with D11344-primed or D8635-primed PCR resulted in DIs of 0.936 and 0.937, respectively. ARDRA revealed a low DI of 0.743 alone but a DI of 0.955 in combination with serotyping. PFGE using the restriction enzyme BglII and opa typing produced the highest discrimination (DIs, 0.997 and 0. 996, respectively) for isolates of N. gonorrhoeae.
选取了一组18株淋病奈瑟菌，这些菌株在时间和地理上具有多样性，用于评估多种分型系统，包括传统的辅助分型和血清分型以及任意引物PCR（AP-PCR）、核糖体DNA扩增限制性分析（ARDRA）、opa分型和脉冲场凝胶电泳（PFGE）等分子方法。采用来自印度尼西亚商业性工作者的87株临床分离株，确定不同分型方法的鉴别能力，并计算辛普森多样性指数。在辅助分型和血清分型这两种传统技术中，后者的鉴别指数（DI）最高（DI为0.846）。辅助分型和血清分型相结合可产生较高的DI（DI为0.928）。以D11344和D8635为引物的PCR的DI分别较低，为0.608和0.622，但两种引物结合后的DI为0.849。血清分型与以D11344或D8635为引物的PCR相结合，DI分别为0.936和0.937。ARDRA单独显示的DI较低，为0.743，但与血清分型结合后的DI为0.955。使用限制性内切酶BglII的PFGE和opa分型对淋病奈瑟菌分离株的鉴别能力最高（DI分别为0.997和0.996）。
