Bilek Nicole, Martin Iona M, Bell Gill, Kinghorn George R, Ison Catherine A, Spratt Brian G
Department of Infectious Disease Epidemiology, Imperial College London, St Mary's Hospital, Norfolk Place, London, UK.
J Clin Microbiol. 2007 Nov;45(11):3564-7. doi: 10.1128/JCM.01453-07. Epub 2007 Sep 12.
Neisseria gonorrhoeae multiantigen sequence typing (NG-MAST) is a highly discriminatory molecular typing procedure that provides precise and unambiguous strain characterization. Since molecular typing can complement contact tracing for reconstructing gonorrhea sexual networks, the concordance between the NG-MAST genotypes of pairs of N. gonorrhoeae isolates from recent sexual contacts was examined. Among 72 pairs of gonococci from recent sexual contacts, the genotypes of each pair were concordant in 65 cases (90.3%). In two further pairs, the isolates from sexual contacts differed by only a single nonsynonymous substitution in the porin gene, and in both of these pairs, the isolates were the same by opa typing. The other five nonconcordant pairs of isolates were clearly different strains. opa typing data were available for 51 of the pairs of isolates from sexual contacts, and concordant opa types were obtained in 38 cases (74.5%). NG-MAST should therefore be better than opa typing at identifying recent sexual contacts and has the important advantage over opa typing of being a more precise method of strain characterization.
淋病奈瑟菌多抗原序列分型（NG-MAST）是一种具有高度鉴别力的分子分型方法，可提供精确且明确的菌株特征描述。由于分子分型可辅助接触者追踪以重建淋病性传播网络，因此对近期性接触中分离出的淋病奈瑟菌菌株对的NG-MAST基因型之间的一致性进行了研究。在72对来自近期性接触的淋球菌中，每对菌株的基因型在65例中一致（90.3%）。另外两对中，性接触分离出的菌株仅在孔蛋白基因中有一个非同义替换差异，且在这两对中，通过opa分型菌株是相同的。其他五对不一致的分离菌株明显是不同的菌株。51对来自性接触的分离菌株有opa分型数据，其中38例（74.5%）获得了一致的opa类型。因此，在识别近期性接触方面，NG-MAST应优于opa分型，并且与opa分型相比具有重要优势，即它是一种更精确的菌株特征描述方法。
