Hinck L W, Ivey M H
J Parasitol. 1976 Oct;62(5):771-4.
Hatching fluid and the excretions and secretions (E.S.) of hatched larvae of Ascaris suum revealed proteinase activity when assayed by 2 different procedures employing collagen or casein as substrates. Both assays apparently detected similar levels of proteinase activity in hatching fluid and E.S. of hatched larvae. The Anson (casein substrate) assay worked best in 0.05 M phosphate buffer, pH 8.0. The Azocoll (collagen substrate) assay worked best in 0.05 M borate buffer at pH 8.8. Azocoll assays done at temperatures ranging from 25 to 65 C revealed maximal proteinase activity at 55 C. Analysis of hatching fluid from 18-, 21-, and 28-day-old embryos and of extracts from sonicated 0- to 28-day-old developmental stages showed that proteinase activity increased markedly 18 days after embryonation had begun. Prior to the 18th day of embryonation proteinase levels were relatively low.
当采用以胶原蛋白或酪蛋白为底物的两种不同方法进行检测时，猪蛔虫的孵化液以及孵化幼虫的排泄物和分泌物（E.S.）显示出蛋白酶活性。两种检测方法显然都检测到了孵化液和孵化幼虫的E.S.中相似水平的蛋白酶活性。安森（酪蛋白底物）检测法在pH 8.0的0.05 M磷酸盐缓冲液中效果最佳。偶氮胶原（胶原蛋白底物）检测法在pH 8.8的0.05 M硼酸盐缓冲液中效果最佳。在25至65℃的温度范围内进行的偶氮胶原检测显示，蛋白酶活性在55℃时达到最大值。对18日龄、21日龄和28日龄胚胎的孵化液以及0至28日龄发育阶段的超声提取物进行分析表明，在胚胎发育开始18天后，蛋白酶活性显著增加。在胚胎发育第18天之前，蛋白酶水平相对较低。
