Blasius W, Leusser K, Merker G
Arzneimittelforschung. 1976;26(9):1645-50.
The effect of O-(beta-hydroxyethyl)-rutosides (Venoruton) on function and structure of the motor anterior-horn cells of the lumbar spinal cord was investigated under conditions of ischemia in the rabbit. The determination of the functional parameters of the ganglion cells, such as maximal function time (Fm), disappearance time (SFm), relative efficiency (La), and regeneration expenditure (Ea) revealed that 50 mg/kg of Venoruton injected prior to repeated aortic occlusion of short duration (occlusion time A = Fm and A = 2 Fm, respectively) caused the efficiency of the anterior-horn cells to be decreased. Both qualitative and quantitative analysis of the mitochondrial structure after prolonged aortic occlusion (15-25 min) revealed that after prior injection of Venoruton irreversible structural changes in the mitochondrial membranes of the ischemic area in the spinal cord occurred after aortic occlusion of 15 min duration. In the untreated controls such changes were not observed before 20 min of occlusion. The mitochondrial structure of the non-ischemic area in the spinal cord was found to be undamaged, however, in both controls and experimental animals. Venoruton given after prolonged aortic occlusion resulted in less pronounced structural changes of the mitochondria in the ischemic area of the experimental animals than those found in the untreated controls; paralysis of the hind extremities was found to occur only after prolonged occlusion (25 min), whereas in the controls such changes were already observed after 20 min of occlusion. Since no ultrastructural changes in the ganglion cells of the non-ischemic area in the in the lumbar spinal cord were observed after application of Venoruton, it is assumed that the decreased efficiency of the motor anterior-horn cells found in the ischemic area when Venoruton had been injected before the aortic occlusion may be due to reactions of the cell that only occur when the blood supply has been completely cut off and Venoruton is present at the same time.
在兔缺血条件下，研究了O-(β-羟乙基)-芦丁（维脑路通）对腰脊髓运动前角细胞功能和结构的影响。对神经节细胞功能参数的测定，如最大功能时间（Fm）、消失时间（SFm）、相对效率（La）和再生消耗（Ea），结果显示，在短时间重复主动脉阻断（阻断时间A分别等于Fm和A等于2Fm）之前注射50mg/kg维脑路通，会导致前角细胞效率降低。对长时间主动脉阻断（15 - 25分钟）后的线粒体结构进行定性和定量分析发现，预先注射维脑路通后，在主动脉阻断15分钟时，脊髓缺血区域线粒体会出现不可逆的结构变化。在未处理的对照组中，在阻断20分钟之前未观察到此类变化。然而，在对照组和实验动物中，脊髓非缺血区域的线粒体结构均未受损。长时间主动脉阻断后给予维脑路通，实验动物缺血区域线粒体的结构变化比未处理的对照组更不明显；仅在长时间阻断（25分钟）后才出现后肢麻痹，而在对照组中，在阻断20分钟后就已观察到此类变化。由于应用维脑路通后，腰脊髓非缺血区域神经节细胞未观察到超微结构变化，因此推测在主动脉阻断前注射维脑路通时，缺血区域运动前角细胞效率降低可能是由于仅在血供完全切断且同时存在维脑路通时细胞才会发生的反应所致。
