Furusawa N
Osaka City University, Faculty of Human Life Science, Japan.
J AOAC Int. 1999 May-Jun;82(3):770-2.
A rapid and simple method for determining residual oxytetracycline (OTC) in chicken products (muscle, liver, and eggs) by high-performance liquid chromatography (HPLC) was developed. Samples were prepared by homogenization with acetonitrile-n-hexane (5 + 4, v/v) followed by centrifugation to minimize fat and protein contents. OTC in the acetonitrile layer was free from interfering compounds when examined by HPLC using a LiChrospher 100 RP-8 end-capped column, a mobile phase of acetonitrile-acetic acid-0.01 M disodium EDTA (28 + 2 + 70, v/v/v), and a photodiode array detector. Average recoveries from samples spiked with OTC (0.1, 0.2, and 1.0 ppm) were > 88%, with coefficients of variation between 2.3 and 5.1%. The limit of detection was 0.05 ppm.
开发了一种通过高效液相色谱法（HPLC）测定鸡肉产品（肌肉、肝脏和鸡蛋）中残留土霉素（OTC）的快速简便方法。样品用乙腈 - 正己烷（5 + 4，v/v）匀浆，然后离心以减少脂肪和蛋白质含量。当使用LiChrospher 100 RP - 8封端柱、乙腈 - 乙酸 - 0.01 M乙二胺四乙酸二钠（28 + 2 + 70，v/v/v）的流动相和光电二极管阵列检测器通过HPLC检测时，乙腈层中的OTC没有干扰化合物。添加OTC（0.1、0.2和1.0 ppm）的样品的平均回收率> 88%，变异系数在2.3%至5.1%之间。检测限为0.05 ppm。
