Gillett N A, Chan C M
Sierra Biomedical, Inc., Sparks, Nevada 89431, USA.
Toxicol Pathol. 1999 Jan-Feb;27(1):48-52. doi: 10.1177/019262339902700110.
Advances in cell and molecular biology have engendered a wide range of techniques that can be used to study the molecular events that underlie the cause of disease, thus producing a new field of study called "molecular pathology." These techniques can be either slide-based or non-slide-based (solution-based). The slide-based techniques include immunohistochemistry, in situ hybridization, and in situ polymerase chain reaction; pathologists play a unique role in the administration of these techniques because of their ability to interpret the end product (i.e., the slide). In this manuscript, we briefly discussed the use and impact of these slide-based techniques within all phases of drug development in the pharmaceutical industry.
细胞与分子生物学的进展催生了一系列可用于研究疾病病因背后分子事件的技术，从而产生了一个名为“分子病理学”的新研究领域。这些技术可以是基于玻片的，也可以是基于溶液的（非玻片型）。基于玻片的技术包括免疫组织化学、原位杂交和原位聚合酶链反应；病理学家在这些技术的应用中发挥着独特作用，因为他们有能力解读最终产物（即玻片）。在本手稿中，我们简要讨论了这些基于玻片的技术在制药行业药物研发各阶段的应用及影响。
