Nakamura M, Inufusa H, Adachi T, Aga M, Kurimoto M, Nakatani Y, Wakano T, Nakajima A, Hida J I, Miyake M, Shindo K, Yasutomi M
The First Department of Surgery, Kinki University School of Medicine, Osakasayama, Osaka 589-8511, Japan.
Int J Oncol. 1999 Jul;15(1):143-8. doi: 10.3892/ijo.15.1.143.
Galectin-3 is a beta-galactoside-specific lectin that binds to laminin sugar-sites and is involved in tumor malignancy. Galectin-3 expression in relation to primary tumor and liver metastasis of colorectal cancer was examined to determined its involvement in cancer progression and metastasis. Immunohistochemical staining of galectin-3 was performed on 117 primary lesions and 15 liver metastases of colorectal cancer using TIB166 monoclonal antibody. The expression of galectin-3 was evaluated by grading the intensity of the staining as either negative, weakly positive, or strongly positive. Normal mucosa of all patients were strongly positive for galectin-3, but the staining in these tissues was still significantly less than in the primary lesions of the cancer (31.6%). Galectin-3 expression in the primary lesions was significantly increased, correlating with the progression of clinical stage (p=0. 0224), liver metastasis (p<0.0001), venous invasion (p=0.0048), and lymph node metastasis (p=0.0289). Liver metastatic lesions also showed up-regulated levels of galectin-3 compared to the primary lesions (p=0.0030). The group showing strongly positive galectin-3 had a significantly poorer prognosis than the negative/weakly positive group in terms of disease-free survival (p=0.0224). The strong expression of galectin-3 in colorectal cancer correlates with cancer progression, liver metastasis, and poor prognosis for patients.
半乳糖凝集素-3是一种β-半乳糖苷特异性凝集素，它与层粘连蛋白糖位点结合并参与肿瘤恶性进展。研究了半乳糖凝集素-3在结直肠癌原发性肿瘤和肝转移中的表达，以确定其在癌症进展和转移中的作用。使用TIB166单克隆抗体对117例结直肠癌原发性病变和15例肝转移灶进行半乳糖凝集素-3的免疫组织化学染色。通过将染色强度分级为阴性、弱阳性或强阳性来评估半乳糖凝集素-3的表达。所有患者的正常黏膜半乳糖凝集素-3呈强阳性，但这些组织中的染色仍明显低于癌症原发性病变（31.6%）。原发性病变中半乳糖凝集素-3的表达显著增加，与临床分期进展（p=0.0224）、肝转移（p<0.0001）、静脉侵犯（p=0.0048）和淋巴结转移（p=0.0289）相关。与原发性病变相比，肝转移灶中半乳糖凝集素-3水平也上调（p=0.0030）。在无病生存期方面，半乳糖凝集素-3呈强阳性的组预后明显比阴性/弱阳性组差（p=0.0224）。结直肠癌中半乳糖凝集素-3的强表达与癌症进展、肝转移以及患者的不良预后相关。
