葡萄糖转运蛋白Hxt1p从内质网的有效输出需要Gsf2p。
Efficient export of the glucose transporter Hxt1p from the endoplasmic reticulum requires Gsf2p.
作者信息
Sherwood P W, Carlson M
机构信息
Department of Genetics and Development, Columbia University, New York, NY 10032, USA.
出版信息
Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7415-20. doi: 10.1073/pnas.96.13.7415.
Abstract
Mutations in the GSF2 gene cause glucose starvation phenotypes in Saccharomyces cerevisiae. We have isolated the HXT1 gene, which encodes a low-affinity, high-capacity glucose transporter, as a multicopy suppressor of a gsf2 mutation. We show that gsf2 mutants accumulate Hxt1p in the endoplasmic reticulum (ER) and that Gsf2p is a 46-kDa integral membrane protein localized to the ER. gsf2 mutants also display a galactose growth defect and abnormal localization of the galactose transporter Gal2p but are not defective in function or localization of the high-affinity glucose transporter Hxt2p. These findings suggest that Gsf2p functions in the ER to promote the secretion of certain hexose transporters.
摘要
GSF2基因突变会导致酿酒酵母出现葡萄糖饥饿表型。我们分离出了HXT1基因，它编码一种低亲和力、高容量的葡萄糖转运蛋白，作为gsf2突变的多拷贝抑制子。我们发现gsf2突变体在内质网（ER）中积累Hxt1p，并且Gsf2p是一种定位于内质网的46 kDa整合膜蛋白。gsf2突变体还表现出半乳糖生长缺陷以及半乳糖转运蛋白Gal2p的定位异常，但高亲和力葡萄糖转运蛋白Hxt2p的功能或定位没有缺陷。这些发现表明，Gsf2p在内质网中发挥作用，促进某些己糖转运蛋白的分泌。
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Proc Natl Acad Sci U S A. 1999 Jun 22;96(13):7415-20. doi: 10.1073/pnas.96.13.7415.
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